Overview

  • Product nameAnti-NQO1 antibody
    See all NQO1 primary antibodies
  • Description
    Goat polyclonal to NQO1
  • SpecificityThis products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1)
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-FoFr, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide: SIPTDNQIKARK, corresponding to C terminal amino acids 267-274 of Human NQO1.

  • Positive control
    • Human kidney, lung and liver lysate
  • General notesPrincipal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab2346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 26-30 kDa.Can be blocked with Human NQO1 peptide (ab22904).
IHC-FoFr 1/250. PubMed: 19186165
ELISA 1/64000.
IHC-Fr Use at an assay dependent dilution. PubMed: 7715346

Target

  • FunctionThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • Sequence similaritiesBelongs to the NAD(P)H dehydrogenase (quinone) family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Azoreductase antibody
    • Cytochrome b 5 reductase antibody
    • DHQU antibody
    • DIA 4 antibody
    • DIA4 antibody
    • Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
    • Diaphorase (NADH/NADPH) antibody
    • Diaphorase 4 antibody
    • Dioxin inducible 1 antibody
    • DT diaphorase antibody
    • DT-diaphorase antibody
    • DTD antibody
    • Menadione reductase antibody
    • NAD(P)H dehydrogenase [quinone] 1 antibody
    • NAD(P)H dehydrogenase quinone 1 antibody
    • NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
    • NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
    • NAD(P)H:menadione oxidoreductase 1 antibody
    • NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
    • NAD(P)H:quinone oxidoreductase 1 antibody
    • NAD(P)H:quinone oxireductase antibody
    • NMOR 1 antibody
    • NMOR I antibody
    • NMOR1 antibody
    • NMORI antibody
    • NQO 1 antibody
    • NQO1 antibody
    • NQO1_HUMAN antibody
    • Phylloquinone reductase antibody
    • QR 1 antibody
    • QR1 antibody
    • Quinone reductase 1 antibody
    see all

Anti-NQO1 antibody images

  • This image was kindly supplied as part of the review submitted by Lauren Aleksunes. Various mouse liver cytosol samples and human recombinant protein were run on the gel and then incubated with ab2346.
  • Anti-NQO1 antibody (ab2346) at 0.03 µg/ml + Human Kidney lysate at 35 µg

    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab2346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2346, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-NQO1 antibody (ab2346)

This product has been referenced in:
  • Eakins R  et al. Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome. Sci Rep 5:16423 (2015). Read more (PubMed: 26607827) »
  • Onodera Y  et al. NRF2 immunolocalization in human breast cancer patients as a prognostic factor. Endocr Relat Cancer 21:241-52 (2014). Human . Read more (PubMed: 24302665) »

See all 26 Publications for this product

Product Wall

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unseres Wissens haben wir noch keine Abbildung von ab2346 Anti-NQO1 antibody in IHC-Fr. Falls Sie uns eine Abbildung Ihrer Ergebnisse mit ab2346 zukommen lasse...

Read More

Thank you for contacting us. I am sorry that ab2346 is giving no signal in WB on rat samples. Based on the protocol you provided, I would recommend trying a primary antibody dilution of 1:1000. Depending on the expression of NQO1 in your samples, you m...

Read More

I'm very sorry to hear you are experiencing problems with lot 105593, we do not have any more vials of lot 44897 or 105593, and if you could provide me with the order details of the vials of lot 105593 I can certainly arrange replacement vials if you h...

Read More

In a situation like this, you can either get a refund (from Novus and we in turn would refund them) or another batch. So I can directly send you another batch of ab2346 once it comes into stock. Please send me your shipping address, including phone num...

Read More

I'm sorry to hear that you are having problems with this antibody. I have contacted the originator of ab2346 and there is a new batch of this antibody with better ELISA results than the previous batch which you received. The new batch has only been tes...

Read More

This antibody has only been tested on mouse liver lysates in Western blot.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"