Anti-NQO1 antibody (ab2346)
Key features and details
- Goat polyclonal to NQO1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-NQO1 antibody
See all NQO1 primary antibodies -
Description
Goat polyclonal to NQO1 -
Host species
Goat -
Specificity
This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1) -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Pig
Predicted to work with: Dog -
Immunogen
Synthetic peptide corresponding to Human NQO1 aa 267-274 (C terminal).
Sequence:C-SIPTDNQIKARK
(Peptide available asab22904) -
Positive control
- WB: U251, HepG2, Human Kidney, Human Lung, Human Duodenum, Rat Kidney, Rat Lung and Rat Duodenum lysates. IHC-P: Human Kidney tissue. ICC/IF: HepG2 cells.
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General notes
Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-32 kDa.
Primary incubation for 1 hour at room temperature. |
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IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 30-32 kDa. Primary incubation for 1 hour at room temperature. |
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. -
Sequence similarities
Belongs to the NAD(P)H dehydrogenase (quinone) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 610935 Dog
- Entrez Gene: 1728 Human
- Entrez Gene: 100286873 Pig
- Entrez Gene: 24314 Rat
- Omim: 125860 Human
- SwissProt: P15559 Human
- SwissProt: P05982 Rat
- Unigene: 406515 Human
see all -
Alternative names
- Azoreductase antibody
- Cytochrome b 5 reductase antibody
- DHQU antibody
see all
Images
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All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : Rat Kidney lysate in RIPA buffer
Lane 2 : Rat Lung lysate in RIPA buffer
Lane 3 : Rat Duodenum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30-32 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : Human Kidney lysate in RIPA buffer
Lane 2 : Human Lung lysate in RIPA buffer
Lane 3 : Human Duodenum lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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All lanes : Anti-NQO1 antibody (ab2346) at 0.3 µg/ml
Lane 1 : U251 cell lysate in RIPA buffer
Lane 2 : HepG2 cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Observed band size: 30 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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ab2346 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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ab2346 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (95)
ab2346 has been referenced in 95 publications.
- Zhengxing X et al. Carbon Monoxide Protects Neural Stem Cells Against Iron Overload by Modulating the Crosstalk Between Nrf2 and NF-κB Signaling. Neurochem Res 47:1383-1394 (2022). PubMed: 35258778
- Mostafazadeh M et al. In vitro exosomal transfer of Nrf2 led to the oxaliplatin resistance in human colorectal cancer LS174T cells. Cell Biochem Funct 40:391-402 (2022). PubMed: 35474580
- Song L et al. Traditional Chinese Medicine Fufang-Zhenzhu-Tiaozhi capsule prevents renal injury in diabetic minipigs with coronary heart disease. Chin Med 17:102 (2022). PubMed: 36042482
- Bondi CD et al. Suppression of NRF2 Activity by HIF-1α Promotes Fibrosis after Ischemic Acute Kidney Injury. Antioxidants (Basel) 11:N/A (2022). PubMed: 36139884
- Horiuchi M et al. Cellular Nrf2 Levels Determine Cell Fate during Chemical Carcinogenesis in Esophageal Epithelium. Mol Cell Biol 41:N/A (2021). PubMed: 33257504