Overview

  • Product nameAnti-NQO1 antibody
    See all NQO1 primary antibodies
  • Description
    Rabbit polyclonal to NQO1
  • Tested applicationsSuitable for: Sandwich ELISA, IHC-P, IHC-Fr, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human NQO1.

    (Peptide available as ab34172.)

  • Positive control
    • This antibody gave a positive signal in the following whole tissue lysates: Human Kidney Normal.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Breast Cancer.

Properties

Applications

Our Abpromise guarantee covers the use of ab34173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA 1/1000.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human NQO1 peptide (ab34172).
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [A180] to NQO1 (ab28947).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
  • Sequence similaritiesBelongs to the NAD(P)H dehydrogenase (quinone) family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Azoreductase antibody
    • Cytochrome b 5 reductase antibody
    • DHQU antibody
    • DIA 4 antibody
    • DIA4 antibody
    • Diaphorase (NADH/NADPH) (cytochrome b 5 reductase) antibody
    • Diaphorase (NADH/NADPH) antibody
    • Diaphorase 4 antibody
    • Dioxin inducible 1 antibody
    • DT diaphorase antibody
    • DT-diaphorase antibody
    • DTD antibody
    • Menadione reductase antibody
    • NAD(P)H dehydrogenase [quinone] 1 antibody
    • NAD(P)H dehydrogenase quinone 1 antibody
    • NAD(P)H menadione oxidoreductase 1 dioxin inducible antibody
    • NAD(P)H: menadione oxidoreductase 1 dioxin inducible 1 antibody
    • NAD(P)H:menadione oxidoreductase 1 antibody
    • NAD(P)H:Quinone acceptor oxidoreductase type 1 antibody
    • NAD(P)H:quinone oxidoreductase 1 antibody
    • NAD(P)H:quinone oxireductase antibody
    • NMOR 1 antibody
    • NMOR I antibody
    • NMOR1 antibody
    • NMORI antibody
    • NQO 1 antibody
    • NQO1 antibody
    • NQO1_HUMAN antibody
    • Phylloquinone reductase antibody
    • QR 1 antibody
    • QR1 antibody
    • Quinone reductase 1 antibody
    see all

Anti-NQO1 antibody images

  • Anti-NQO1 antibody (ab34173) at 1 µg/ml + Human Kidney Whole Tissue Lysate at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti Rabbit IgG (H&L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa
  • Image courtesy of Human Protein Atlas . ab34173 staining NQO1 in human breast cancer. Paraffin embedded human breast cancer tissue was incubated with ab34173 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab34173 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab34173 stained HeLa cells. The cells were 4% formaldehdye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34173, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Sandwich ELISA for the detection of NQO1, using ab28947 (1/500) as the cature antibody and ab34173 (1/1000) for the detection. Please refer to abreview for further experimental details.

    See Abreview

References for Anti-NQO1 antibody (ab34173)

This product has been referenced in:
  • Greco T  et al. Ketogenic diet decreases oxidative stress and improves mitochondrial respiratory complex activity. J Cereb Blood Flow Metab 36:1603-13 (2016). Read more (PubMed: 26661201) »
  • Crilly MJ  et al. The role of Nrf2 in skeletal muscle contractile and mitochondrial function. J Appl Physiol (1985) 121:730-40 (2016). Read more (PubMed: 27471236) »

See all 14 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (ovary)
Antigen retrieval step Enzymatic
Permeabilization No
Specification ovary
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.5%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 02 2015

Yes, based on the immunogen this antibody should be able to pick up all three isoforms on the NQO1 protein.



Thank you for contacting us.

I couldn't find the dog sequence on Swiss Prot, so unfortunately I couldn't BLAST the sequences to compare them for you. The immunogen is from a.a. XXX - XXX so if you have access to the sequence, you may be able...

Read More

Thank you very much for supplying that information.

I am sorry to hear of your experience with ab34173. As requested, I am trying to track down which antibody was used in the paper you provided. However this is likely to be difficult as we su...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Specification Heart
Fixative Paraformaldehyde
Permeabilization Yes - it is an nuclear protein
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 30°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

Submitted Dec 08 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart, Skeletal muscle)
Loading amount 30 µg
Specification Heart, Skeletal muscle
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 30°C
Username

Mr. Mtuhtu S.Gounder

Verified customer

Submitted Nov 30 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (kidney (HK2))
Loading amount 10 µg
Specification kidney (HK2)
Gel Running Conditions Reduced Denaturing (4-12 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Mar 15 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1)
Loading amount 10 µg
Specification THP-1
Gel Running Conditions Reduced Denaturing (5-20% gradient)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Oct 28 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Pancreas, Pancreatic ductal adenocarcinoma)
Specification Pancreas, Pancreatic ductal adenocarcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid, pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 04 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Liver, colon, duodenum)
Loading amount 50 µg
Specification Liver, colon, duodenum
Gel Running Conditions Reduced Denaturing (gel 12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Aug 25 2009

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"