Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human NQO1.
(Peptide available as ab34172.)
Our Abpromise guarantee covers the use of ab34173 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).Can be blocked with Human NQO1 peptide (ab34172).|
|ELISA||Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [A180] to NQO1 (ab28947).|
|ICC/IF||Use a concentration of 1 µg/ml.|
Image courtesy of Human Protein Atlas . ab34173 staining NQO1 in human breast cancer. Paraffin embedded human breast cancer tissue was incubated with ab34173 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab34173 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org