Overview

  • Product nameAnti-NQO2 antibody
    See all NQO2 primary antibodies
  • Description
    Rabbit polyclonal to NQO2
  • Tested applicationsSuitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) conjugated to KLH, selected from the Center region of NQO2.

  • Positive control
    • Mouse liver tissue lysate IF/ICC: HeLa cell line.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab93211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
ELISA
  • Application notesELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 26 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
    • Sequence similaritiesBelongs to the NAD(P)H dehydrogenase (quinone) family.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • DHQV antibody
      • DIA6 antibody
      • EC 1.10.99.2 antibody
      • MGC94180 antibody
      • NAD(P)H dehydrogenase quinone 2 antibody
      • NAD(P)H menadione oxidoreductase 1 dioxin inducible 2 antibody
      • NAD(P)H menadione oxidoreductase 2 dioxin inducible antibody
      • NMOR2 antibody
      • NQO 2 antibody
      • NQO2 antibody
      • NQO2_HUMAN antibody
      • NRH dehydrogenase [quinone] 2 antibody
      • NRH dehydrogenase antibody
      • NRH:quinone oxidoreductase 2 antibody
      • OTTHUMP00000015948 antibody
      • OTTHUMP00000015949 antibody
      • OTTHUMP00000015953 antibody
      • Ox 2 antibody
      • Ox2 antibody
      • QR2 antibody
      • Quinone antibody
      • Quinone reductase 2 antibody
      • Ribosyldihydronicotinamide dehydrogenase [quinone] antibody
      • Ribosyldihydronicotinamide dehydrogenase antibody
      see all

    Anti-NQO2 antibody images

    • ICC/IF image of ab93211 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93211, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Anti-NQO2 antibody (ab93211) at 1/100 dilution + Mouse liver tissue lysate at 35 µg

      Predicted band size : 26 kDa

    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 26 kDa


      Exposure time : 8 minutes

    References for Anti-NQO2 antibody (ab93211)

    ab93211 has not yet been referenced specifically in any publications.

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