The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
1/140. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Belongs to the NAD(P)H dehydrogenase (quinone) family.
Flow Cytometric analysis of C6 cells labeling NQO2 with ab181049 at 1/140 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Western blot - Anti-NQO2 [EPR13345] antibody (ab181049)
All lanes : Anti-NQO2 antibody [EPR13345] (ab181049) at 1/5000 dilution
Lane 1 : L6 cell lysate Lane 2 : C2C12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution