Recombinant
RabMAb

Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)

Overview

  • Product name
    Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal
    See all NR0B1 / Dax1 primary antibodies
  • Description
    Rabbit monoclonal [EP13786] to NR0B1 / Dax1 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NR0B1/ Dax1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P51843

  • Positive control
    • A431 and Human testis lysates; Human testis tissue; SH-SY5Y cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab196649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/300.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Flow Cyt Use at an assay dependent concentration.

Target

  • Function
    Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency.
  • Involvement in disease
    Defects in NR0B1 are the cause of X-linked adrenal hypoplasia congenital (XL-AHC) [MIM:300200]; also known as X-linked Addison disease (AHX). XL-AHC is a developmental disorder of the adrenal gland that results in profound hormonal deficiencies and is lethal if untreated. It is characterized by the absence of the permanent zone of the adrenal cortex and by a structural disorganization of the glands. Hypogonadotropic hypogonadism (HHG) is frequently associated with this disorder. HHG is a condition resulting from or characterized by abnormally decreased gonadal function, with retardation of growth and sexual development.
    Defects in NR0B1 are the cause of 46,XY sex reversal type 2 (SRXY2) [MIM:300018]. It is a condition characterized by male-to-female sex reversal in the presence of a normal 46,XY karyotype. Note=XY individuals with a duplication of part of the short arm of the X chromosome and an intact SRY gene develop as females. The single X chromosome in these individuals does not undergo X-chromosome inactivation; therefore, these individuals presumably carry 2 active copies of genes, including the NR0B1 gene, in the duplicated region. Individuals with deletion of this region develop as males. Genes within the dosage-sensitive sex reversal region are, therefore, not essential for testis development, but, when present in a double dose, interfere with testis formation.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR0 subfamily.
  • Domain
    Homodimerization involved an interaction between amino and carboxy termini involving LXXLL motifs and steroid binding domain (AF-2 motif). Heterodimerizes with NR5A1 and NROB2 through its N-terminal LXXLL motifs.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the cytoplasm and nucleus. Homodimers exits in the cytoplasm and in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHC antibody
    • AHCH antibody
    • AHX antibody
    • DAX 1 antibody
    • DAX1 antibody
    • Dosage sensitive sex reversal antibody
    • DSS antibody
    • DSS AHC critical region on the X chromosome protein 1 antibody
    • DSS-AHC critical region on the X chromosome protein 1 antibody
    • GTD antibody
    • HHG antibody
    • Nr0b1 antibody
    • NR0B1_HUMAN antibody
    • NROB1 antibody
    • Nuclear hormone receptor antibody
    • Nuclear receptor 0B1 antibody
    • Nuclear receptor DAX 1 antibody
    • Nuclear receptor DAX-1 antibody
    • Nuclear receptor DAX1 antibody
    • Nuclear receptor subfamily 0 group B member 1 antibody
    • SRXY2 antibody
    see all

Images

  • Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling NR0B1 / Dax1 with purified ab196649 at 1:150 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunofluorescent analysis of SH-SY5Y cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NR0B1 / Dax1 with ab196649 at 1/300 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

    Note: Nuclear and cytoplasm staining on SH-SY5Y cell line was observed.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NR0B1 / Dax1 with ab196649 at 1/1000 dilution  followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).

    Note: Nuclear staining on human testis tissue was observed.

  • Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 52 kDa

  • Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + A431 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 52 kDa

References

ab196649 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (Adrenal gland, HAC15 adrenocortical cell line)
Negative control
Normal Rabbit IgG processed sample
Specification
Adrenal gland, HAC15 adrenocortical cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: FORMALDEHYDE 40%, Final formal
Positive control
Nuclear lysate which has been processed with antibody against Histone H3 (D2B12)XP rabbit mAb (ChIP formulated) (different company, no Abcam), following the same protocol as Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649).
Username

Abcam user community

Verified customer

Submitted Aug 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic Stem Cells)
Permeabilization
Yes - 0.2% Tritonx
Specification
Embryonic Stem Cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Simon Hastreiter

Verified customer

Submitted Aug 15 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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