Overview

  • Product nameAnti-NR0B2 antibody
    See all NR0B2 primary antibodies
  • Description
    Rabbit polyclonal to NR0B2
  • Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cat, Dog, Chimpanzee, Orangutan, Spider Monkey
  • Immunogen

    Synthetic peptide: EVAEAPVPSI LKKILLEEPS SSGGSGQLPD RPQPSLAAVQ WLQCCLESFW , corresponding to internal sequence amino acids 108-157 of Human NR0B2

  • Positive control
    • Transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab80694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

Target

  • FunctionActs as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.
  • Tissue specificityLiver. Low levels of expression were detected in heart and pancreas.
  • Involvement in diseaseObesity
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR0 subfamily.
  • Post-translational
    modifications
    Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
  • Cellular localizationNucleus. Cytoplasm. Colocalizes with NEUROD1 in the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • NR0B2 antibody
    • NR0B2_HUMAN antibody
    • Nr0b2a antibody
    • Nuclear receptor subfamily 0 group B member 2 antibody
    • Nuclear receptor subfamily 0, group B, member 2a antibody
    • Orphan nuclear receptor SHP antibody
    • SHP antibody
    • SHP-1 antibody
    • Shp1 antibody
    • Small heterodimer partner antibody
    see all

Anti-NR0B2 antibody images

  • Anti-NR0B2 antibody (ab80694) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Gel concentration 12%
    Detects a few other paler bands running up to ~75kDa.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling NR0B2 with ab80694 at 5.0µg/ml.

References for Anti-NR0B2 antibody (ab80694)

ab80694 has not yet been referenced specifically in any publications.

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