Anti-NR0B2 antibody [N7519] (ab41947)

Overview

  • Product nameAnti-NR0B2 antibody [N7519]
    See all NR0B2 primary antibodies
  • Description
    Mouse monoclonal [N7519] to NR0B2
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    E.coli expressed recombinant full length protein (Human) NR0B2

Properties

Applications

Our Abpromise guarantee covers the use of ab41947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at a concentration of 0.5 µg/ml.
    WB: Use at a concentration of 3 µg/ml, under non reducing or reducing conditions. Predicted molecular weight: 29 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActs as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated trancription complex for NEUROD1.
    • Tissue specificityLiver. Low levels of expression were detected in heart and pancreas.
    • Involvement in diseaseObesity
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR0 subfamily.
    • Post-translational
      modifications
      Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
    • Cellular localizationNucleus. Cytoplasm. Colocalizes with NEUROD1 in the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • NR0B2 antibody
      • NR0B2_HUMAN antibody
      • Nr0b2a antibody
      • Nuclear receptor subfamily 0 group B member 2 antibody
      • Nuclear receptor subfamily 0, group B, member 2a antibody
      • Orphan nuclear receptor SHP antibody
      • SHP antibody
      • SHP-1 antibody
      • Shp1 antibody
      • Small heterodimer partner antibody
      see all

    References for Anti-NR0B2 antibody [N7519] (ab41947)

    ab41947 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - nuclear (Human hepatocytes in culture)
    Loading amount 10 µg
    Specification Human hepatocytes in culture
    Treatment 50uM chenodeoxycholic acid
    Gel Running Conditions Reduced Denaturing (15% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

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    Verified customer

    Submitted Nov 12 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"