Overview

  • Product name
    Anti-NR1D1 antibody [4F6]
    See all NR1D1 primary antibodies
  • Description
    Mouse monoclonal [4F6] to NR1D1
  • Tested applications
    Suitable for: WB, Sandwich ELISA, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein with proprietary tag, corresponding to amino acids 1-615 of Human NR1D1 ((AAH56148).

  • Positive control
    • Hela nuclear extract; NIH3T3 cell lysate; HeLa cells; Human Brain, Cortex tissue.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab115552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 67 kDa.
Sandwich ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.01µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Functions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.
  • Tissue specificity
    Expressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAR-1 antibody
    • EAR1 antibody
    • ERBA-related 1 antibody
    • hRev antibody
    • Nr1d1 antibody
    • NR1D1_HUMAN antibody
    • Nuclear receptor Rev ErbA alpha antibody
    • Nuclear receptor subfamily 1 group D member 1 antibody
    • Rev erbAalpha antibody
    • Rev erbalpha antibody
    • Rev-erbA-alpha antibody
    • Rev-ErbAalpha antibody
    • Reverba antibody
    • THRA1 antibody
    • THRAL antibody
    • Thyroid hormone receptor, alpha like antibody
    • Thyroid hormone receptor, alpha-1- like antibody
    • V-erbA-related protein 1 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab115552 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115552, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-NR1D1 antibody [4F6] (ab115552) + HeLa nuclear extract

    Predicted band size : 67 kDa
  • Anti-NR1D1 antibody [4F6] (ab115552) + NIH3T3 cell lysate

    Predicted band size : 67 kDa
  • ab115552, at 5µg/ml, staining NR1D1 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115552 at 10µg/ml dilution staining NR1D1 in HeLa cells by Immunofluorescence.
  • Detection limit for recombinant tagged NR1D1 is approximately 1 ng/ml as a capture antibody.

References

This product has been referenced in:
  • Mukherji A  et al. Shifting eating to the circadian rest phase misaligns the peripheral clocks with the master SCN clock and leads to a metabolic syndrome. Proc Natl Acad Sci U S A 112:E6691-8 (2015). Read more (PubMed: 26627260) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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