Overview

  • Product nameAnti-NR1D1 antibody [4F6]
    See all NR1D1 primary antibodies
  • Description
    Mouse monoclonal [4F6] to NR1D1
  • Tested applicationsSuitable for: WB, Sandwich ELISA, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein with proprietary tag, corresponding to amino acids 1-615 of Human NR1D1 ((AAH56148).

  • Positive control
    • Hela nuclear extract; NIH3T3 cell lysate; HeLa cells; Human Brain, Cortex tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab115552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 67 kDa.
Sandwich ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 0.01µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionFunctions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.
  • Tissue specificityExpressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAR-1 antibody
    • EAR1 antibody
    • ERBA-related 1 antibody
    • hRev antibody
    • Nr1d1 antibody
    • NR1D1_HUMAN antibody
    • Nuclear receptor Rev ErbA alpha antibody
    • Nuclear receptor subfamily 1 group D member 1 antibody
    • Rev erbAalpha antibody
    • Rev erbalpha antibody
    • Rev-erbA-alpha antibody
    • Rev-ErbAalpha antibody
    • Reverba antibody
    • THRA1 antibody
    • THRAL antibody
    • Thyroid hormone receptor, alpha like antibody
    • Thyroid hormone receptor, alpha-1- like antibody
    • V-erbA-related protein 1 antibody
    see all

Anti-NR1D1 antibody [4F6] images

  • Overlay histogram showing HeLa cells stained with ab115552 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115552, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Anti-NR1D1 antibody [4F6] (ab115552) + HeLa nuclear extract

    Predicted band size : 67 kDa
  • Anti-NR1D1 antibody [4F6] (ab115552) + NIH3T3 cell lysate

    Predicted band size : 67 kDa
  • ab115552, at 5µg/ml, staining NR1D1 in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115552 at 10µg/ml dilution staining NR1D1 in HeLa cells by Immunofluorescence.
  • Detection limit for recombinant tagged NR1D1 is approximately 1 ng/ml as a capture antibody.

References for Anti-NR1D1 antibody [4F6] (ab115552)

ab115552 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"