The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at a concentration of 0.01 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml under reducing or non reducing conditions. Predicted molecular weight: 67 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionFunctions as a constitutive transcriptional repressor. Possible receptor for triiodothyronine.
Tissue specificityExpressed in all tissues and cell lines examined. Expressed at high levels in some squamous carcinoma cell lines.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain.
DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.