Overview

  • Product name
  • Description
    Rabbit polyclonal to NR1D1
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: VISQVARAHR EIFTYAHDKL GSSPGNFNAN HASGSPPATT PHRWENQGCP , corresponding to internal sequence amino acids 287-336 of Human NR1D1

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab80697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1/312500.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 67 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-NR1D1 antibody (ab80697) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 67 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References

ab80697 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80697.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up