Overview

  • Product nameAnti-NR1D2 antibody
    See all NR1D2 primary antibodies
  • Description
    Mouse monoclonal to NR1D2
  • Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-580 of Human NR1D2

Properties

Applications

Our Abpromise guarantee covers the use of ab56913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionBinds to the sequences 5'-AATGTAGGTCA-3' and 5'-ATAACTAGGTCA-3'. Acts as a potent competitive repressor of ROR alpha function.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • BD73 antibody
    • EAR 1R antibody
    • EAR-1R antibody
    • HZF2 antibody
    • Nr1d2 antibody
    • NR1D2_HUMAN antibody
    • Nuclear receptor Rev ErbA beta variant 1 antibody
    • Nuclear receptor Rev ErbA beta variant 2 antibody
    • Nuclear receptor subfamily 1 group D member 2 antibody
    • Orphan nuclear hormone receptor BD73 antibody
    • Rev erb beta antibody
    • Rev erba alpha related receptor antibody
    • Rev-erb-beta antibody
    • REVB antibody
    • REVERBB antibody
    • RVR antibody
    • V-erbA-related protein 1-related antibody
    see all

Anti-NR1D2 antibody images

  • NR1D2 antibody (ab56913) used in immunofluorescence at 10ug/ml on HeLa cells.
  • NR1D2 antibody (ab56913) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing HeLa cells stained with ab56913 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56913, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-NR1D2 antibody (ab56913)

ab56913 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (HEK 293A)
Loading amount 50 µg
Specification HEK 293A
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted May 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"