Nuclear receptor subfamily 1 group D member 2 antibody
Orphan nuclear hormone receptor BD73 antibody
Rev erb beta antibody
Rev erba alpha related receptor antibody
V-erbA-related protein 1-related antibody
Anti-NR1D2 antibody images
Immunofluorescence - NR1D2 antibody (ab56913)
NR1D2 antibody (ab56913) used in immunofluorescence at 10ug/ml on HeLa cells.
Western blot - NR1D2 antibody (ab56913)
NR1D2 antibody (ab56913) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry-Anti-NR1D2 antibody(ab56913)
Overlay histogram showing HeLa cells stained with ab56913 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56913, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-NR1D2 antibody (ab56913)
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