Overview

  • Product name
  • Description
    Rabbit polyclonal to NR2C2
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: HLQNSIQEDK LSGDRIKQVM EHIWKLQEFC NSMAKLDIDG YEYAYLKAIV, corresponding to a region within C terminal amino acids 469-518 of Human NR2C2

  • Positive control
    • HepG2 cell lysate. Human skin.

Properties

Applications

Our Abpromise guarantee covers the use of ab65676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 8 µg/ml.
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Developmental stage
    Transiently repressed during the meiotic phase of spermatogenesis.
  • Post-translational
    modifications
    Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Form
    There are 2 isoforms produced by alternative splicing.
  • Alternative names
    • hTAK1 antibody
    • Nr2c2 antibody
    • NR2C2_HUMAN antibody
    • Nuclear hormone receptor TR4 antibody
    • Nuclear receptor subfamily 2 group C member 2 antibody
    • Orphan nuclear receptor TAK1 antibody
    • Orphan nuclear receptor TR4 antibody
    • TAK1 antibody
    • Testicular nuclear receptor 4 antibody
    • Testicular receptor 4 antibody
    • TR2R1 antibody
    • TR4 antibody
    • TR4 nuclear hormone receptor antibody
    see all

Images

  • Anti-NR2C2 antibody (ab65676) at 2.5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • Immunohistochemical analysis of paraffin-embedded human skin tissue stained with ab65676 at 4µg/ml. Positively stained squamous epithelial cells are indicated with arrows.

References

ab65676 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab65676.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up