Anti-NR2C2 antibody [EPR1772(2)] (ab109513)

Overview

  • Product nameAnti-NR2C2 antibody [EPR1772(2)]
    See all NR2C2 primary antibodies
  • Description
    Rabbit monoclonal [EPR1772(2)] to NR2C2
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human NR2C2.

  • Positive control
    • 293T, PC-3 and U937 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 65 kDa).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • FunctionOrphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Developmental stageTransiently repressed during the meiotic phase of spermatogenesis.
    • Post-translational
      modifications
      Phosphorylation on Ser-19 and Ser-68 is an important regulator of NR2C2-mediated transcriptional activity. Phosphorylation on these residues recruits the corepressor, NRIP1, leading to transcripional repression, whereas the nonphosphorylated form preferentially recruits the coactivator, PCAF.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • FormThere are 2 isoforms produced by alternative splicing.
    • Alternative names
      • hTAK1 antibody
      • Nr2c2 antibody
      • NR2C2_HUMAN antibody
      • Nuclear hormone receptor TR4 antibody
      • Nuclear receptor subfamily 2 group C member 2 antibody
      • Orphan nuclear receptor TAK1 antibody
      • Orphan nuclear receptor TR4 antibody
      • TAK1 antibody
      • Testicular nuclear receptor 4 antibody
      • Testicular receptor 4 antibody
      • TR2R1 antibody
      • TR4 antibody
      • TR4 nuclear hormone receptor antibody
      see all

    Anti-NR2C2 antibody [EPR1772(2)] images

    • All lanes : Anti-NR2C2 antibody [EPR1772(2)] (ab109513) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : PC-3 cell lysate
      Lane 3 : U937 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 65 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)

    References for Anti-NR2C2 antibody [EPR1772(2)] (ab109513)

    ab109513 has not yet been referenced specifically in any publications.

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