Recombinant Anti-NR2E1/Tailless/TLX antibody [EPR4491] (ab109179)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4491] to NR2E1/Tailless/TLX
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-NR2E1/Tailless/TLX antibody [EPR4491]
See all NR2E1/Tailless/TLX primary antibodies -
Description
Rabbit monoclonal [EPR4491] to NR2E1/Tailless/TLX -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Fetal brain, Human hippocampus, SH-SY5Y and Jurkat lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 9.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4491 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa. |
Target
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Function
Orphan receptor that binds DNA as a monomer to hormone response elements (HRE) containing an extended core motif half-site sequence 5'-AAGGTCA-3' in which the 5' flanking nucleotides participate in determining receptor specificity. May be required for brain development. May be involved in the regulation of retinal development. -
Tissue specificity
Brain specific. Present in all brain sections tested, highest levels in the caudate nucleus and hippocampus, weakest levels in the thalamus. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7101 Human
- Entrez Gene: 21907 Mouse
- Entrez Gene: 684085 Rat
- Omim: 603849 Human
- SwissProt: Q9Y466 Human
- SwissProt: Q3UXE8 Mouse
- SwissProt: Q64104 Mouse
- SwissProt: Q78ZM1 Mouse
see all -
Alternative names
- hTll antibody
- NR2E1 / Tailless antibody
- nr2e1 antibody
see all
Images
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All lanes : Anti-NR2E1/Tailless/TLX antibody [EPR4491] (ab109179) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Human hippocampus lysate
Lane 3 : SH-SY5Y lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109179 has been referenced in 2 publications.
- Tian H et al. Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration. iScience 25:105050 (2022). PubMed: 36185374
- Dueva E et al. Computer-Aided Discovery of Small Molecule Inhibitors of Transcriptional Activity of TLX (NR2E1) Nuclear Receptor. Molecules 23:N/A (2018). PubMed: 30441799