Anti-NR2E3 antibody [H7223] (ab41922)

Overview

  • Product nameAnti-NR2E3 antibody [H7223]
    See all NR2E3 primary antibodies
  • Description
    Mouse monoclonal [H7223] to NR2E3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide: ETRPTALMSS TVAAAAPAAG AASRKESPGR WGLGEDPTG VSPSL, corresponding to amino acids 2-45 of Human NR2E3

  • Positive control
    • Human and rat eye retina.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPhysiological saline. Preservative: 0.1% Sodium Azide
  • Concentration information loading...
  • Purification notesPurified from ascites via ammonium sulfate fractionation.
  • ClonalityMonoclonal
  • Clone numberH7223
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.
ELISA Use a concentration of 0.001 µg/ml.
IHC-P Use a concentration of 10 - 20 µg/ml.

Target

  • FunctionOrphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M-and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M- and S-cone opsin expression.
  • Tissue specificityEye specific; found solely in the outer nuclear layer of the adult neurosensory retina, where the nuclei of cone and rod photoreceptors reside.
  • Involvement in diseaseDefects in NR2E3 are a cause of enhanced S cone syndrome (ESCS) [MIM:268100]. ESCS is an autosomal recessive retinopathy in which patients have increased sensitivity to blue light; perception of blue light is mediated by what is normally the least populous cone photoreceptor subtype, the S (short wavelength, blue) cones. ESCS is also associated with visual loss, with night blindness occurring from early in life, varying degrees of L (long, red)- and M (middle, green)-cone vision, and retinal degeneration.
    Defects in NR2E3 are the cause of retinitis pigmentosa type 37 (RP37) [MIM:611131]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP37 inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Post-translational
    modifications
    Di- and tri-sumoylated in developing retina. PIAS3-mediated sumoylation promotes repression of cone-specific gene expression and activation of rod-specific genes. Sumoylation on Lys-185 appears to be the main site.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ESCS antibody
    • MGC49976 antibody
    • NR2 E3 antibody
    • Nr2e3 antibody
    • NR2E3_HUMAN antibody
    • Nuclear receptor subfamily 2 group E member 3 antibody
    • Photoreceptor specific nuclear receptor antibody
    • Photoreceptor-specific nuclear receptor antibody
    • PNR antibody
    • Rd 7 antibody
    • Rd7 antibody
    • Retina specific nuclear receptor antibody
    • Retina-specific nuclear receptor antibody
    • Retinal degeneration 7 antibody
    • RNR antibody
    • RP37 antibody
    see all

Anti-NR2E3 antibody [H7223] images

  • ab41922 staining NR2E3 in human eye retina, outer nuclear layer (10-20ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.
  • ab41922 staining NR2E3 in rat eye retina, outer nuclear layer (10-20ug/mL) by immunohistochemistry, formalin-fixed paraffin embedded sections.

References for Anti-NR2E3 antibody [H7223] (ab41922)

ab41922 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (retina)
Specification retina
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton x
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Mr. Kevin Yoon

Verified customer

Submitted Feb 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"