Overview

  • Product nameAnti-NR2F6 antibody
    See all NR2F6 primary antibodies
  • Description
    Rabbit polyclonal to NR2F6
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog
  • Immunogen

    A region within synthetic peptide: PLHTAPLLAA AGLHAAPMAA ERAVAFMDQV RAFQEQVDKL GRLQVDSAEY, corresponding to internal sequence amino acids 253-302 of Human NR2F6

  • Positive control
    • HepG2 cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:1562500.

WB Use a concentration of 5 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionTranscription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
  • Tissue specificityExpressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAR 2 antibody
    • EAR-2 antibody
    • EAR2 antibody
    • ERBA RELATED 2 antibody
    • ERBA related gene 2 antibody
    • ERBAL2 antibody
    • Nr2f6 antibody
    • NR2F6_HUMAN antibody
    • Nuclear receptor subfamily 2 group F member 6 antibody
    • Orphan nuclear receptor EAR2 (V erbA related protein EAR 2) antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like antibody
    • V erbA related protein EAR 2 antibody
    • V-erbA-related protein 2 antibody
    see all

Anti-NR2F6 antibody images

  • Anti-NR2F6 antibody (ab49222) at 5 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

References for Anti-NR2F6 antibody (ab49222)

ab49222 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"