Overview

  • Product name
  • Description
    Rabbit polyclonal to NR2F6
  • Tested applications
    Suitable for: ELISA, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from N-terminal of human NR2F6.

  • Positive control
    • HeLa, 293, HUVEC or HepG2 cells. Cervix carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab65012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
  • Tissue specificity
    Expressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EAR 2 antibody
    • EAR-2 antibody
    • EAR2 antibody
    • ERBA RELATED 2 antibody
    • ERBA related gene 2 antibody
    • ERBAL2 antibody
    • Nr2f6 antibody
    • NR2F6_HUMAN antibody
    • Nuclear receptor subfamily 2 group F member 6 antibody
    • Orphan nuclear receptor EAR2 (V erbA related protein EAR 2) antibody
    • v erb a avian erythroblastic leukemia viral oncogene homolog like antibody
    • V erbA related protein EAR 2 antibody
    • V-erbA-related protein 2 antibody
    see all

Images

  • ab65012 at 1/500 - 1/1000 dilution staining NR2F6 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • ab65012 at 1/50 - 1/100 dilution staining human cervix carcinoma in NR2F6 by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-NR2F6 antibody (ab65012) at 1/500 dilution

    Lane 1 : HeLa cell extract at 10-30µg
    Lane 2 : 293 cell extract at 10-30µg
    Lane 3 : HUVEC cell extract at 10-30µg
    Lane 4 : HUVEC cell extract at 10-30µg with immunizing peptide at 5-10µg


    Predicted band size : 43 kDa
    Observed band size : 43 kDa

References

This product has been referenced in:
  • Weatherford ET  et al. Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6. Am J Physiol Renal Physiol 302:F1025-33 (2012). WB . Read more (PubMed: 22278040) »
  • Modica S  et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology 138:636-48, 648.e1-12 (2010). IHC-P ; Human . Read more (PubMed: 19818784) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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