The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
1/500 - 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
FunctionTranscription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
Tissue specificityExpressed in heart, placenta, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain.
ab65012 at 1/50 - 1/100 dilution staining human cervix carcinoma in NR2F6 by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
Western blot - NR2F6 antibody (ab65012)
All lanes : Anti-NR2F6 antibody (ab65012) at 1/500 dilution
Lane 1 : HeLa cell extract at 10-30µg Lane 2 : 293 cell extract at 10-30µg Lane 3 : HUVEC cell extract at 10-30µg Lane 4 : HUVEC cell extract at 10-30µg with immunizing peptide at 5-10µg
Predicted band size : 43 kDa Observed band size : 43 kDa
References for Anti-NR2F6 antibody (ab65012)
This product has been referenced in:
Weatherford ET et al. Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6. Am J Physiol Renal Physiol302:F1025-33 (2012).
Read more (PubMed: 22278040) »
Modica S et al. The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors. Gastroenterology138:636-48, 648.e1-12 (2010).
Read more (PubMed: 19818784) »