Overview

  • Product name
    Anti-NR5A2 / LRH1 antibody
    See all NR5A2 / LRH1 primary antibodies
  • Description
    Goat polyclonal to NR5A2 / LRH1
  • Host species
    Goat
  • Specificity
    ab223211 is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human, Pig
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide corresponding to Human NR5A2/ LRH1 aa 233-246 (internal sequence) (Cysteine residue). (NP_995582.1).
    Sequence:

    C-TDYDRSPFVTSPIS


    Database link: O00482

  • Positive control
    • WB: HepG2 nuclear lysate; Pig ovary lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab223211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 61 kDa).

Target

  • Function
    Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
  • Tissue specificity
    Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-1-fetoprotein transcription factor antibody
    • B1-binding factor antibody
    • B1F antibody
    • B1F2 antibody
    • CPF antibody
    • CYP7A promoter-binding factor antibody
    • FTF antibody
    • FTZ F1 antibody
    • FTZ F1beta antibody
    • FTZ-F1 antibody
    • FTZ-F1beta antibody
    • hB1F 2 antibody
    • hB1F antibody
    • hB1F-2 antibody
    • Hepatocytic transcription factor antibody
    • Liver receptor homolog 1 antibody
    • LRH-1 antibody
    • LRH1 antibody
    • Nr5a2 antibody
    • NR5A2_HUMAN antibody
    • Nuclear receptor subfamily 5 group A member 2 antibody
    see all

Images

  • Anti-NR5A2 / LRH1 antibody (ab223211) at 1 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) nuclear lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 55 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

  • Anti-NR5A2 / LRH1 antibody (ab223211) at 0.3 µg/ml + Pig ovary lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 61 kDa
    Observed band size: 60 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab223211 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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