Overview

  • Product name
    Anti-NR5A2 / LRH1 antibody
    See all NR5A2 / LRH1 primary antibodies
  • Description
    Rabbit polyclonal to NR5A2 / LRH1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Sheep, Guinea pig, Cow, Pig, Chimpanzee
  • Immunogen

    A region within synthetic peptide: MSSNSDTGDL QESLKHGLTP IVSQFKMVNY SYDEDLEELC PVCGDKVSGY, corresponding to N terminal amino acids 1-50 of Human NR5A2/ LRH1

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab62601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/12500.

Target

  • Function
    Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
  • Tissue specificity
    Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-1-fetoprotein transcription factor antibody
    • B1-binding factor antibody
    • B1F antibody
    • B1F2 antibody
    • CPF antibody
    • CYP7A promoter-binding factor antibody
    • FTF antibody
    • FTZ F1 antibody
    • FTZ F1beta antibody
    • FTZ-F1 antibody
    • FTZ-F1beta antibody
    • hB1F 2 antibody
    • hB1F antibody
    • hB1F-2 antibody
    • Hepatocytic transcription factor antibody
    • Liver receptor homolog 1 antibody
    • LRH-1 antibody
    • LRH1 antibody
    • Nr5a2 antibody
    • NR5A2_HUMAN antibody
    • Nuclear receptor subfamily 5 group A member 2 antibody
    see all

Images

  • Lane 1 : Marker
    Lane 2 : Anti-NR5A2 / LRH1 antibody (ab62601) at 2.5 µg/ml

    Lane 1 : As above
    Lane 2 : HepG2 cell lysate at 10 µg

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)

References

ab62601 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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