Recombinant
RabMAb

Anti-NR5A2 / LRH1 antibody [EPR7000] (ab125034)

Overview

  • Product name
    Anti-NR5A2 / LRH1 antibody [EPR7000]
    See all NR5A2 / LRH1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7000] to NR5A2 / LRH1
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human NR5A2/ LRH1.

  • Positive control
    • HeLa, BxPC3, HepG2 and MCF7 cell lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab125034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 61 kDa).Can be blocked with NR5A2 / LRH1 peptide (ab169741).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function
      Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
    • Tissue specificity
      Abundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
    • Sequence similarities
      Belongs to the nuclear hormone receptor family. NR5 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha-1-fetoprotein transcription factor antibody
      • B1-binding factor antibody
      • B1F antibody
      • B1F2 antibody
      • CPF antibody
      • CYP7A promoter-binding factor antibody
      • FTF antibody
      • FTZ F1 antibody
      • FTZ F1beta antibody
      • FTZ-F1 antibody
      • FTZ-F1beta antibody
      • hB1F 2 antibody
      • hB1F antibody
      • hB1F-2 antibody
      • Hepatocytic transcription factor antibody
      • Liver receptor homolog 1 antibody
      • LRH-1 antibody
      • LRH1 antibody
      • Nr5a2 antibody
      • NR5A2_HUMAN antibody
      • Nuclear receptor subfamily 5 group A member 2 antibody
      see all

    Images

    • All lanes : Anti-NR5A2 / LRH1 antibody [EPR7000] (ab125034) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : BxPC3 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 61 kDa
      Observed band size : 65 kDa (why is the actual band size different from the predicted?)

    References

    ab125034 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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