• Product nameAnti-NR5A2 / LRH1 antibody [H2325]
    See all NR5A2 / LRH1 primary antibodies
  • Description
    Mouse monoclonal [H2325] to NR5A2 / LRH1
  • SpecificityThis antibody specifically recognizes human LRH1 alpha and beta and cross reacts with mouse and rat LRH1 alpha and beta.
  • Tested applicationsSuitable for: WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Baculovirus-expressed recombinant fragment, corresponding to amino acids 161-280 of Human NR5A2/ LRH1



Our Abpromise guarantee covers the use of ab41901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa.
ELISA Use a concentration of 0.002 µg/ml.
IP Use at an assay dependent dilution.


  • FunctionBinds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development.
  • Tissue specificityAbundantly expressed in pancreas, less in liver, very low levels in heart and lung. Expressed in the Hep-G2 cell line. Isoform 1 and isoform 2 seem to be present in fetal and adult liver and Hep-G2 cells.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR5 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-1-fetoprotein transcription factor antibody
    • B1-binding factor antibody
    • B1F antibody
    • B1F2 antibody
    • CPF antibody
    • CYP7A promoter-binding factor antibody
    • FTF antibody
    • FTZ F1 antibody
    • FTZ F1beta antibody
    • FTZ-F1 antibody
    • FTZ-F1beta antibody
    • hB1F 2 antibody
    • hB1F antibody
    • hB1F-2 antibody
    • Hepatocytic transcription factor antibody
    • Liver receptor homolog 1 antibody
    • LRH-1 antibody
    • LRH1 antibody
    • Nr5a2 antibody
    • NR5A2_HUMAN antibody
    • Nuclear receptor subfamily 5 group A member 2 antibody
    see all

Anti-NR5A2 / LRH1 antibody [H2325] images

  • All lanes : Anti-NR5A2 / LRH1 antibody [H2325] (ab41901) at 1/1000 dilution

    Lane 1 : COS-1 cells transfected with NR5A2
    Lane 2 : COS-1 cells transfected with NR5A2
    Lane 3 : COS-1 cells transfected with NR5A2
    Lane 4 : COS-1 cells transfected with NR5A2
    Lane 5 : COS-1 cells transfected with NR5A2
    Lane 6 : COS-1 cells transfected with NR5A2
    Lane 7 : COS-1 cells transfected with NR5A2
    Lane 8 : COS-1 cells transfected with NR5A2

    Lysates/proteins at 15 µg per lane.

    HRP-conjugated Goat anti-Mouse IgG polyclonal at 1/2500 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Observed band size : 61 kDa

    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Chun Fai Lai.

    See Abreview

References for Anti-NR5A2 / LRH1 antibody [H2325] (ab41901)

This product has been referenced in:
  • Duggan SP  et al. The characterization of an intestine-like genomic signature maintained during Barrett's-associated adenocarcinogenesis reveals an NR5A2-mediated promotion of cancer cell survival. Sci Rep 6:32638 (2016). WB ; Human . Read more (PubMed: 27586588) »
  • Baquié M  et al. The liver receptor homolog-1 (LRH-1) is expressed in human islets and protects {beta}-cells against stress-induced apoptosis. Hum Mol Genet 20:2823-33 (2011). ICC/IF ; Human . Read more (PubMed: 21536586) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (COS-1)
Loading amount 15 µg
Specification COS-1
Treatment transfected with NR5A2
Gel Running Conditions Reduced Denaturing (10% Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Mr. Chun Fui Lai

Verified customer

Submitted Feb 15 2011