Overview

  • Product nameAnti-NRAMP1 antibody
    See all NRAMP1 primary antibodies
  • Description
    Rabbit polyclonal to NRAMP1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Sheep, Rabbit, Goat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: FARVLLTRSC AILPTVLVAV FRDLRDLSGL NDLLNVLQSL LLPFAVLPIL , corresponding to amino acids 398-447 of Human NRAMP1

  • Positive control
    • HepG2 membrane lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab59696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

Target

  • FunctionDivalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.
  • Tissue specificityMacrophages; peripheral blood leukocytes, lung, spleen and liver.
  • Sequence similaritiesBelongs to the NRAMP family.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LSH antibody
    • Natural resistance associated macrophage protein 1 antibody
    • Natural resistance-associated macrophage protein 1 antibody
    • NRAM1_HUMAN antibody
    • NRAMP 1 antibody
    • NRAMP antibody
    • PBC antibody
    • SLC11A1 antibody
    • Solute carrier family 11 (proton coupled divalent metal ion transporters) member 1 antibody
    • solute carrier family 11 (sodium/phosphate symporters) member 1 antibody
    • Solute carrier family 11 member 1 antibody
    see all

Anti-NRAMP1 antibody images

  • Anti-NRAMP1 antibody (ab59696) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 60 kDa
    Observed band size : 60 kDa

References for Anti-NRAMP1 antibody (ab59696)

ab59696 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Rat Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 17 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jun 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 50 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 50 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Liver)
Loading amount 50 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Sep 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HepG2)
Loading amount 20 µg
Specification HepG2
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Shakil Ahmad

Verified customer

Submitted Aug 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"