Anti-NRAP antibody (ab122427)
Key features and details
- Rabbit polyclonal to NRAP
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NRAP antibody -
Description
Rabbit polyclonal to NRAP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
internal sequence amino acids 1309-1393 (RHDFVKERGK LIGPQSVRDD PRIQHCRRMG QLQSELQYRR GATSSQAQFH LPMDMVHLVH AKNAQALASD HDYRTQYHKF TALPE) of the Human NRAP protein (UniProt ID: Q86VF7).
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Positive control
- IHC-P: Human skeletal muscle and heart muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix. -
Tissue specificity
Expressed in cardiac and skeletal muscle. -
Sequence similarities
Contains 1 LIM zinc-binding domain.
Contains 44 nebulin repeats. -
Cellular localization
Localized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle. - Information by UniProt
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Database links
- Entrez Gene: 4892 Human
- Omim: 602873 Human
- SwissProt: Q86VF7 Human
- Unigene: 268788 Human
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Alternative names
- N-RAP antibody
- Nebulin-related-anchoring protein antibody
- Nrap antibody
- NRAP_HUMAN antibody
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart muscle tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling NRAP with ab122427 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab122427 has been referenced in 2 publications.
- Li F et al. Expressing a Z-disk nebulin fragment in nebulin-deficient mouse muscle: effects on muscle structure and function. Skelet Muscle 10:2 (2020). PubMed: 31992366
- Truszkowska GT et al. Homozygous truncating mutation in NRAP gene identified by whole exome sequencing in a patient with dilated cardiomyopathy. Sci Rep 7:3362 (2017). WB ; Human . PubMed: 28611399