Overview

  • Product nameAnti-NRAP antibody
    See all NRAP primary antibodies
  • Description
    Rabbit polyclonal to NRAP
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Sheep, Human
  • Immunogen

    internal sequence amino acids 1309-1393 (RHDFVKERGK LIGPQSVRDD PRIQHCRRMG QLQSELQYRR GATSSQAQFH LPMDMVHLVH AKNAQALASD HDYRTQYHKF TALPE) of the Human NRAP protein (UniProt ID: Q86VF7).

  • Positive control
    • Human liver tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab122427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • FunctionMay be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix.
  • Tissue specificityExpressed in cardiac and skeletal muscle.
  • Sequence similaritiesContains 1 LIM zinc-binding domain.
    Contains 44 nebulin repeats.
  • Cellular localizationLocalized at the myotendinous junction in skeletal muscle and at the intercalated disk in cardiac muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • N-RAP antibody
    • Nebulin-related-anchoring protein antibody
    • Nrap antibody
    • NRAP_HUMAN antibody

Anti-NRAP antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli, centrosome and cell junctions. Recommended concentration of ab122427 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122427, at a 1/200 dilution, staining NRAP in paraffin embedded Human liver tissue by Immunohistochemistry.
  • Anti-NRAP antibody (ab122427) at 1/1000 dilution + Sheep cardiac tissue at 10 µg

    Secondary
    Donkey Anti-Rabbit IgG (H+L) at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 190 kDa (why is the actual band size different from the predicted?)


    Exposure time : 50 seconds

    This image is courtesy of an anonymous abreview.

    See Abreview

References for Anti-NRAP antibody (ab122427)

ab122427 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Sheep Tissue lysate - other (cardiac)
Specification cardiac
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Dec 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"