Overview

  • Product name
  • Description
    Goat polyclonal to NRAS
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Dog, Pig, Opossum, Orangutan
  • Immunogen

    Synthetic peptide:

    C-RMKKLNSSDDGTQ

    , corresponding to internal sequence amino acids 167-179 of Human NRAS (NP_002515.1).

  • Positive control
    • Human Small Intestine tissue and A431 lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab118814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Predicted molecular weight: 21 kDa.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in disease
    Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications
    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Images

  • ab118814, at 2.5 µg/ml, staining NRAS in formalin-fixed, paraffin-embedded Human Small Intestine tissue by Immunohistochemistry using a biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-NRAS antibody (ab118814) at 0.01 µg/ml + A431 lysate in RIPA buffer at 35 µg

    Predicted band size : 21 kDa

References

ab118814 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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