Overview

  • Product nameAnti-NRAS antibody
    See all NRAS primary antibodies
  • Description
    Goat polyclonal to NRAS
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Guinea pig, Cow, Dog, Pig, Orangutan
  • Immunogen

    Synthetic peptide:

    C-RMKKLNSSDDGTQ

    , corresponding to internal sequence amino acids 167-179 of Human NRAS (NP_002515.1).

  • Positive control
    • A431 lysate, human small intestine, rat intestine.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab77392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 - 3 µg/ml.
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in diseaseDefects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications
    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
  • Cellular localizationCell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Anti-NRAS antibody images

  • Anti-NRAS antibody (ab77392) at 0.01 µg/ml + A431 lysate (in RIPA buffer) at 35 µg

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
  • ab77392 at 2.5 µg/ml staining NRAS in human small intestine tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • Anti-NRAS antibody (ab77392) at 0.1 µg/ml + Rat intestine lysate in RIPA buffer. at 35 µg
    Developed using the ECL technique

    Predicted band size : 21 kDa
    Observed band size : 20 kDa (why is the actual band size different from the predicted?)

References for Anti-NRAS antibody (ab77392)

This product has been referenced in:
  • Sun D  et al. RAS/MEK-independent gene expression reveals BMP2-related malignant phenotypes in the Nf1-deficient MPNST. Mol Cancer Res 11:616-27 (2013). Read more (PubMed: 23423222) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"