Overview

  • Product name
    Anti-NRAS antibody [AT2G9]
    See all NRAS primary antibodies
  • Description
    Mouse monoclonal [AT2G9] to NRAS
  • Specificity
    ab113627 recognises HRAS, NRAS and KRAS.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-186 of Human NRAS purified from E. coli (NP_002515).

  • Positive control
    • Recombinant NRAS, KRAS and HRAS, 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab113627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/5000. Predicted molecular weight: 21 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function
    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • Involvement in disease
    Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
    Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Ras family.
  • Post-translational
    modifications
    Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
  • Cellular localization
    Cell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPS4 antibody
    • AV095280 antibody
    • GTPase NRas antibody
    • HRAS1 antibody
    • N ras antibody
    • N ras protein part 4 antibody
    • Neuroblastoma RAS viral (v ras) oncogene homolog antibody
    • NRAS antibody
    • NRAS1 antibody
    • NS6 antibody
    • OTTHUMP00000013879 antibody
    • OTTMUSP00000023521 antibody
    • RASN_HUMAN antibody
    • Transforming protein N Ras antibody
    • Transforming protein N-Ras antibody
    • v ras neuroblastoma RAS viral oncogene homolog antibody
    see all

Images

  • Lane 1 : Anti-NRAS antibody [AT2G9] (ab113627) at 1/500 dilution
    Lane 2 : Anti-NRAS antibody [AT2G9] (ab113627) at 1/1000 dilution
    Lane 3 : Anti-NRAS antibody [AT2G9] (ab113627) at 1/5000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 21 kDa
  • All lanes : Anti-NRAS antibody [AT2G9] (ab113627) at 1/500 dilution

    Lane 1 : recombinant NRAS
    Lane 2 : recombinant KRAS
    Lane 3 : recombinant HRAS

    Lysates/proteins at 0.2 µg per lane.


    Predicted band size : 21 kDa

References

ab113627 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab113627.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up