All lanes : Anti-NRBF2 antibody (ab85733) at 0.4 µg/ml
Lane 1 : Whole cell
lysate from HeLa cells at 50 µg Lane 2 : Whole cell
lysate from HeLa cells at 15 µg Lane 3 : Whole cell
lysate from HeLa cells at 5 µg Lane 4 : Whole cell
lysate from 293T cells at 50 µg Lane 5 : Whole cell
lysate from NIH3T3 cells at 50 µg
Detection of Human NRBF2 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85733 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
References for Anti-NRBF2 antibody (ab85733)
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