Overview

  • Product name
  • Description
    Rabbit polyclonal to NRBF2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 237 and 287 of Human NRBF2 (NP_110386.2).

  • Positive control
    • Whole cell lysate from HeLa, 293T and mouse NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab85733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 32 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro).
  • Tissue specificity
    Detected in keratinocytes, liver and placenta.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Comodulator of PPAR and RXR 1 antibody
    • Comodulator of PPAR and RXR 2 antibody
    • Comodulator of PPAR and RXR antibody
    • COPR antibody
    • COPR1 antibody
    • COPR2 antibody
    • DKFZp564C1664 antibody
    • FLJ30395 antibody
    • NRBF 2 antibody
    • NRBF-2 antibody
    • NRBF2 antibody
    • NRBF2_HUMAN antibody
    • Nuclear receptor binding factor 2 antibody
    • Nuclear receptor-binding factor 2 antibody
    see all

Images

  • All lanes : Anti-NRBF2 antibody (ab85733) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Human NRBF2 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85733 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab85733 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain)
Permeabilization
Yes - triton X-100
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.2% · Temperature: RT°C
Fixative
Formaldehyde
Username

Xiaosen Ouyang

Verified customer

Submitted Dec 08 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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