mouse NrCAM protein as fusion with human Fc region of Ig. This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region
Our Abpromise guarantee covers the use of ab24344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||1/200. Do not perform antigen retrieval.|
|WB||1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 145 kDa).|
|IP||Use at an assay dependent concentration.|
|IHC-FoFr||1/200. PubMed: 113319561/200. Tissue was immersion fixed (not perfusion fixed) overnight in 4% paraformaldehyde/PBS pH 7.4 and then cryoprotected by incubation in 30% sucrose overnight. Sections were blocked for 1 hour with 1% goat serum/5% BSA in PBS with 0.02% sodium azide and 0.02% Triton X-100 (see PubMed: 11331956).|
This image is courtesy of Martin Grumet, Rutgers University, United Statesab24344 also can be used for immunoprecipatation and blocks function