Anti-NrCAM antibody - Neuronal Marker (ab24344)

Overview

  • Product name
    Anti-NrCAM antibody - Neuronal Marker
    See all NrCAM primary antibodies
  • Description
    Rabbit polyclonal to NrCAM - Neuronal Marker
  • Specificity
    ab24344 immunoblots a single major protein of NrCAM (see western blot image on datasheet) and recognizes proteins of comparable size from mouse, rat and human.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, WB, IP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    mouse NrCAM protein as fusion with human Fc region of Ig. This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region

  • General notes
    This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region.

Properties

Applications

Our Abpromise guarantee covers the use of ab24344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/200. Do not perform antigen retrieval.
ICC/IF 1/500.
WB 1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 145 kDa).
IP Use at an assay dependent concentration.
IHC-FoFr 1/200. PubMed: 113319561/200. Tissue was immersion fixed (not perfusion fixed) overnight in 4% paraformaldehyde/PBS pH 7.4 and then cryoprotected by incubation in 30% sucrose overnight. Sections were blocked for 1 hour with 1% goat serum/5% BSA in PBS with 0.02% sodium azide and 0.02% Triton X-100 (see PubMed: 11331956).

Target

  • Function
    Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.
  • Tissue specificity
    Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bravo antibody
    • hBravo antibody
    • Neuronal cell adhesion molecule antibody
    • Neuronal surface protein Bravo antibody
    • Ng CAM related antibody
    • Ng-CAM-related antibody
    • NgCAM related cell adhesion molecule antibody
    • NgCAM-related cell adhesion molecule antibody
    • Nr CAM antibody
    • Nr-CAM antibody
    • Nrcam antibody
    • NRCAM_HUMAN antibody
    see all

Images

  • ab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50umab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50um
  • Anti-NrCAM antibody - Neuronal Marker (ab24344) at 1/500 dilution + 20 µg of postnatal day 8 mouse brain extract in SDS

    Predicted band size : 145 kDa
    Observed band size : 145 kDa

    This image is courtesy of Martin Grumet, Rutgers University, United States

    ab24344 also can be used for immunoprecipatation and blocks function

References

This product has been referenced in:
  • Morelli KH  et al. Severity of Demyelinating and Axonal Neuropathy Mouse Models Is Modified by Genes Affecting Structure and Function of Peripheral Nodes. Cell Rep 18:3178-3191 (2017). WB, IHC ; Mouse . Read more (PubMed: 28355569) »
  • Martin PM  et al. Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier. J Biol Chem 292:2441-2456 (2017). Read more (PubMed: 27979964) »

See all 21 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Fluorescent assisted cell sorting)
Specification
Fluorescent assisted cell sorting
Preparation
Cell harvesting/tissue preparation method: Cortex digested with Accutase, debris filtered via percoll gradient, cells strained through a 40uM filter and then stained with 1Ab
Sample buffer: RNAse free PBS
Fixation
No fixation
Permeabilization
No
Gating Strategy
neuronal cells were gated
Username

Abcam user community

Verified customer

Submitted Mar 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Retina)
Specification
Retina
Fixative
Paraformaldehyde
Blocking step
Other as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
Username

Abcam user community

Verified customer

Submitted Aug 01 2007

Thank you for your enquiry. For further information about the protein sequence, please refer to the publication below. http://www.jcb.org/cgi/reprint/113/6/1399 Please bear in mind that this product has not been tested in flow cytometry and ...

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Yes, Karen and I spoke and decided on the advice Karen gave to you. I have still have the same comments. What results did you obtain from the no primary control you ran? If you are still seeing the high background with the secondary only, have you tr...

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Thank you for your reply and attaching the images. You have a very poor result but at this point I have no way of determining if the antibody is causing the problem or not. It would be highly unusual that an antibody would yield a pattern of staining...

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I'm sorry to hear you are having a problem with ab24344. I would like to suggest the following modifications to your protocol: Please use a fresh blot and try: blocking in 5% milk for 30 min, incubating in primary overnight in 5% milk at 4 degrees...

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