Anti-NrCAM antibody - Neuronal Marker (ab24344)

Overview

  • Product name
    Anti-NrCAM antibody - Neuronal Marker
    See all NrCAM primary antibodies
  • Description
    Rabbit polyclonal to NrCAM - Neuronal Marker
  • Host species
    Rabbit
  • Specificity
    ab24344 immunoblots a single major protein of NrCAM (see western blot image on datasheet) and recognizes proteins of comparable size from mouse, rat and human.
  • Tested applications
    Suitable for: IHC-Fr, IHC-P, ICC/IF, WB, IP, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    mouse NrCAM protein as fusion with human Fc region of Ig. This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region

  • General notes
    This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region.

Properties

Applications

Our Abpromise guarantee covers the use of ab24344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/200. Do not perform antigen retrieval.
ICC/IF 1/500.
WB 1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 145 kDa).
IP Use at an assay dependent concentration.
IHC-FoFr 1/200. PubMed: 113319561/200. Tissue was immersion fixed (not perfusion fixed) overnight in 4% paraformaldehyde/PBS pH 7.4 and then cryoprotected by incubation in 30% sucrose overnight. Sections were blocked for 1 hour with 1% goat serum/5% BSA in PBS with 0.02% sodium azide and 0.02% Triton X-100 (see PubMed: 11331956).

Target

  • Function
    Cell adhesion, ankyrin-binding protein involved in neuron-neuron adhesion. May play a role in the molecular assembly of the nodes of Ranvier.
  • Tissue specificity
    Detected in all the examined tissues. In the brain it was detected in the amygdala, caudate nucleus, corpus callosum, hippocampus, hypothalamus, substantia nigra, subthalamic nucleus and thalamus.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bravo antibody
    • hBravo antibody
    • Neuronal cell adhesion molecule antibody
    • Neuronal surface protein Bravo antibody
    • Ng CAM related antibody
    • Ng-CAM-related antibody
    • NgCAM related cell adhesion molecule antibody
    • NgCAM-related cell adhesion molecule antibody
    • Nr CAM antibody
    • Nr-CAM antibody
    • Nrcam antibody
    • NRCAM_HUMAN antibody
    see all

Images

  • ab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50um

    ab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50um
  • Anti-NrCAM antibody - Neuronal Marker (ab24344) at 1/500 dilution + 20 µg of postnatal day 8 mouse brain extract in SDS

    Predicted band size: 145 kDa
    Observed band size: 145 kDa



    ab24344 also can be used for immunoprecipatation and blocks function

References

This product has been referenced in:
  • Morelli KH  et al. Severity of Demyelinating and Axonal Neuropathy Mouse Models Is Modified by Genes Affecting Structure and Function of Peripheral Nodes. Cell Rep 18:3178-3191 (2017). WB, IHC ; Mouse . Read more (PubMed: 28355569) »
  • Martin PM  et al. Schwannomin-interacting Protein 1 Isoform IQCJ-SCHIP1 Is a Multipartner Ankyrin- and Spectrin-binding Protein Involved in the Organization of Nodes of Ranvier. J Biol Chem 292:2441-2456 (2017). Read more (PubMed: 27979964) »

See all 21 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. For further information about the protein sequence, please refer to the publication below. http://www.jcb.org/cgi/reprint/113/6/1399 Please bear in mind that this product has not been tested in flow cytometry and ...

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Yes, Karen and I spoke and decided on the advice Karen gave to you. I have still have the same comments. What results did you obtain from the no primary control you ran? If you are still seeing the high background with the secondary only, have you tr...

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Thank you for your reply and attaching the images. You have a very poor result but at this point I have no way of determining if the antibody is causing the problem or not. It would be highly unusual that an antibody would yield a pattern of staining...

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I'm sorry to hear you are having a problem with ab24344. I would like to suggest the following modifications to your protocol: Please use a fresh blot and try: blocking in 5% milk for 30 min, incubating in primary overnight in 5% milk at 4 degrees...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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