The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 10 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
FunctionTranscription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Tissue specificityUbiquitously expressed with strongest expression in skeletal muscle.
Sequence similaritiesBelongs to the NRF1/Ewg family.
Post-translational modificationsPhosphorylation enhances DNA binding.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling NRF1 with ab86516 at 10µg/ml.
References for Anti-NRF1 antibody (ab86516)
This product has been referenced in:
Rivalin R et al. The NFL-TBS.40-63 anti-glioblastoma peptide disrupts microtubule and mitochondrial networks in the T98G glioma cell line. PLoS One9:e98473 (2014).
Read more (PubMed: 24896268) »