Overview

  • Product name
  • Description
    Rabbit polyclonal to NRF1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 359-408 (WATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNS E) of Human NRF1 (NP_005002)

  • Positive control

Properties

Applications

Our Abpromise guarantee covers the use of ab86516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-NRF1 antibody (ab86516) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 54 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling NRF1 with ab86516 at 10µg/ml.

References

This product has been referenced in:
  • Chung E  et al. Maternal exercise upregulates mitochondrial gene expression and increases enzyme activity of fetal mouse hearts. Physiol Rep 5:N/A (2017). WB ; Mouse . Read more (PubMed: 28292876) »
  • Meparishvili M  et al. A Proteomic Study of Memory After Imprinting in the Domestic Chick. Front Behav Neurosci 9:319 (2015). WB . Read more (PubMed: 26635566) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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