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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Overview
Product name
Anti-NRF1 antibody - ChIP Grade
See all NRF1 products (4) ...
Description
Rabbit polyclonal to NRF1 - ChIP Grade
Specificity
ab34682 recognises NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal).
Tested applications
Gel supershift assays, ChIP, WB, IHC-P, ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Chicken, Dog, Chimpanzee, Zebrafish
Immunogen
Recombinant full length protein, corresponding to amino acids 1-534 of Mouse NRF1
Positive control
HeLa Whole Cell Lysate HeLa Cell Nuclear Extract Human testis
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GSA: Use at an assay dependent concentration. (PubMed: 21609478)
ChIP: Use at an assay dependent dilution.
WB: 1/500 - 1/2000.Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa).(Splice variants exist for this protein that may result in the detection of lower molecular weight bands.)
IHC-P: Use a concentration of 2 - 5 µg/ml.
ICC/IF: Use a concentration of 1 µg/ml
ELISA: 1/3000 - 1/10,000.
Target
Function
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
Tissue specificity
Ubiquitously expressed with strongest expression in skeletal muscle.
Sequence similarities
Belongs to the NRF1/Ewg family.
Post-translational
modifications
Phosphorylation enhances DNA binding.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ16656
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- alpha pal antibody
- alpha palindromic binding protein antibody
- Alpha palindromic-binding protein antibody
see all
Database links
- Entrez Gene: 416677 Chicken
- Entrez Gene: 4899 Human
- Entrez Gene: 18181 Mouse
- Entrez Gene: 312195 Rat
- Entrez Gene: 64604 Zebrafish
- Omim: 600879 Human
- SwissProt: Q16656 Human
- SwissProt: Q9CXE4 Mouse
see all
Anti-NRF1 antibody - ChIP Grade images:
Western blot - NRF1 antibody (ab34682)
All lanes : Anti-NRF1 antibody - ChIP Grade (ab34682) at 1/500 dilution
Lane 1 : HeLa Nuclear extract
Lane 2 : HeLa Whole Cell lysate
Secondary
IRDyeTM700 labelled goat-a-rabbit IgG at 1/2500 dilution
Predicted band size : 57 kDa
Observed band size : 67 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - NRF1 antibody (ab34682)
Purified anti-NRF1 antibody (ab34682) was used at 5µg/ml to detect NRF1 in human testis. Nuclear staining in lymphocytes can be observed.
Immunocytochemistry/ Immunofluorescence-NRF1 antibody - ChIP Grade(ab34682)
ICC/IF image of ab34682 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34682, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-NRF1 antibody - ChIP Grade (ab34682)
This product has been referenced in:
- Shin JHet al. PARIS (ZNF746) repression of PGC-1a contributes to neurodegeneration in Parkinson's disease. Cell 144:689-702 (2011).Read more (PubMed: 21376232) »
- Thompson Cet al. Decreased expression of BRCA1 in SK-BR-3 cells is the result of aberrant activation of the GABP Beta promoter by an NRF-1-containing complex. Mol Cancer 10:62 (2011). ChIP, Gel supershift assays.Read more (PubMed: 21609478) »
See all 8 publications for this product
Publishing research using ab34682? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"