Anti-Nrf2 antibody [437C2a] (ab54364)


  • Product name
    Anti-Nrf2 antibody [437C2a]
    See all Nrf2 primary antibodies
  • Description
    Mouse monoclonal [437C2a] to Nrf2
  • Tested applications
    Suitable for: WB, ICC/IF, Dot blotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Nrf2 recombinant fragment (Human)



Our Abpromise guarantee covers the use of ab54364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 68 kDa. Detects a band of approximately 40 kDa for the recombinant fragment.
ICC/IF Use a concentration of 5 µg/ml.
Dot blot Use at an assay dependent dilution.


  • Function
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • Tissue specificity
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • Sequence similarities
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • Domain
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • Post-translational
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Anti-Nrf2 antibody [437C2a] images

  • Anti-Nrf2 antibody [437C2a] (ab54364) + immunised recombinant protein

    Predicted band size : 68 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab54364 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54364, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Nrf2 antibody [437C2a] (ab54364)

ab54364 has not yet been referenced specifically in any publications.

Product Wall

Ab54364 is actually provided in a dilute solution at 0.1 mg/mL, so it might be necessary to use a more concentrated primary antibody solution. I'd suggest using a 1:100 (1 ug/mL) or 1:50 dilution (2 ug/mL) to see if the results improve.

Thank you again for contacting us and letting us know about this trouble.

We are sending a vial of ab53019 on the order ***.

Please let me know if you have any questions or if there is anything else that we can do for you.

Thank you for contacting us and for letting us know about the trouble with ab54364. I am sorry that this antibody did not work as expected.

Wewill be happy to send a free of charge vial of ab53019 in exchange for ab54364. I have asked my coll...

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