Overview

  • Product name
  • Description
    Goat polyclonal to Nrf2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide:

    C-KHSSRLEAHLTRDE

    with a Cysteine residue linker, corresponding to internal sequence amino acids 445-458 of Human Nrf2 (NP_006155.2; NP_001138884.1; NP_001138885.1).

  • Positive control
    • Human lung lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab121035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).

Target

  • Function
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • Tissue specificity
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • Sequence similarities
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • Domain
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • Post-translational
    modifications
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all

Images

  • Anti-Nrf2 antibody (ab121035) at 0.3 µg/ml + Human lung lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 68 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References

ab121035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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