Anti-Nrf2 antibody - Aminoterminal end (ab75919)


  • Product name
    Anti-Nrf2 antibody - Aminoterminal end
    See all Nrf2 primary antibodies
  • Description
    Rabbit polyclonal to Nrf2 - Aminoterminal end
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Nrf2 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q16236

  • Positive control
    • Ramos cell line lysates and 293 cell lysates either nontransfected or transiently transfected with the Nrf2 gene. IF/ICC: HeLa cell line



Our Abpromise guarantee covers the use of ab75919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/50 - 1/100. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
ELISA 1/1000.


  • Function
    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.
  • Tissue specificity
    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
  • Sequence similarities
    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.
  • Domain
    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.
  • Post-translational
    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.
  • Cellular localization
    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • erythroid derived 2 antibody
    • HEBP1 antibody
    • like 2 antibody
    • NF E2 related factor 2 antibody
    • NF-E2-related factor 2 antibody
    • NF2L2_HUMAN antibody
    • NFE2 related factor 2 antibody
    • NFE2-related factor 2 antibody
    • Nfe2l2 antibody
    • Nrf 2 antibody
    • NRF2 antibody
    • Nuclear factor (erythroid derived 2) like 2 antibody
    • Nuclear factor antibody
    • nuclear factor erythroid 2 like 2 antibody
    • Nuclear factor erythroid 2 related factor 2 antibody
    • Nuclear factor erythroid 2-related factor 2 antibody
    • Nuclear factor erythroid derived 2 like 2 antibody
    see all


  • ICC/IF image of ab75919 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75919, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Nrf2 antibody - Aminoterminal end (ab75919) at 1/60 dilution + Ramos cell line lysates at 35 µg

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 28 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-Nrf2 antibody - Aminoterminal end (ab75919) at 1/50 dilution

    Lane 1 : 293 cell lysates, nontransfected
    Lane 2 : 293 cell lysates, transiently transfected with the Nrf2 gene

    Lysates/proteins at 2 µg per lane.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa


ab75919 has not yet been referenced specifically in any publications.

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Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Mouse Tissue lysate - whole (Liver)
Loading amount
10 µg
partial hepatectomy.
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Dr. Yuhong Zou

Verified customer

Submitted Jan 30 2012


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