Anti-NRG1 antibody [7D5] (ab2369)

Overview

  • Product nameAnti-NRG1 antibody [7D5]
    See all NRG1 primary antibodies
  • Description
    Mouse monoclonal [7D5] to NRG1
  • Tested applicationsSuitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant extracellular domain of rat NRG1 (Neuregulin 1) protein.

  • Positive control
    • Prostate carcinoma
  • General notes


    NRG1 (Neuregulin 1) binds to e-erbB3 and c-erbB4 receptors on mammary and neuronal cells with low and high affinity, respectively. NRG1 exists in several isoforms, which are classified in two groups, a and b, that differ in their EGF-like domain and in receptor binding affinity. This antibody detects pan-NRG1 and is not subtype specific.

Properties

Applications

Our Abpromise guarantee covers the use of ab2369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/50 - 1/100. ABC method.

Target

  • FunctionDirect ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.
  • Tissue specificityType I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific.
  • Involvement in diseaseNote=A chromosomal aberration involving NRG1 produces gamma-heregulin. Translocation t(8;11) with ODZ4. The translocation fuses the 5'-end of ODZ4 to NRG1 (isoform 8). The product of this translocation was first thought to be an alternatively spliced isoform. Gamma-heregulin is a soluble activating ligand for the ERBB2-ERBB3 receptor complex and acts as an autocrine growth factor in a specific breast cancer cell line (MDA-MB-175). Not detected in breast carcinoma samples, including ductal, lobular, medullary, and mucinous histological types, neither in other breast cancer cell lines.
  • Sequence similaritiesBelongs to the neuregulin family.
    Contains 1 EGF-like domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • Developmental stageDetectable at early embryonic ages. Isoform 10 is highly expressed in developing spinal motor neurons and in developing cranial nerve nuclei. Expression is maintained only in both adult motor neurons and dorsal root ganglion neurons. Type IV isoforms are expressed in fetal brain.
  • DomainThe cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
    ERBB receptor binding is elicited entirely by the EGF-like domain.
  • Post-translational
    modifications
    Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
    N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
  • Cellular localizationSecreted; Cell membrane. Does not seem to be active; Membrane. May possess an internal uncleaved signal sequence; Nucleus. May be nuclear and Secreted. Has a signal peptide.
  • Information by UniProt
  • Database links
  • Alternative names
    • Acetylcholine receptor-inducing activity antibody
    • Acetylcholine receptor-inducing activity, CHICK, homolog of antibody
    • ARIA antibody
    • Breast cancer cell differentiation factor p45 antibody
    • GGF antibody
    • GGF2 antibody
    • Glial growth factor antibody
    • Heregulin antibody
    • heregulin, alpha (45kD, ERBB2 p185-activator) antibody
    • HGL antibody
    • HRG antibody
    • HRG1 antibody
    • HRGA antibody
    • MST131 antibody
    • NDF antibody
    • Neu differentiation factor antibody
    • Neuregulin 1 antibody
    • neuregulin 1 type IV beta 1a antibody
    • neuregulin 1 type IV beta 3 antibody
    • Neuregulin-1 antibody
    • NRG 1 antibody
    • nrg1 antibody
    • NRG1-IT2 antibody
    • NRG1_HUMAN antibody
    • OTTHUMP00000225398 antibody
    • OTTHUMP00000225419 antibody
    • OTTHUMP00000225420 antibody
    • OTTHUMP00000225421 antibody
    • OTTHUMP00000225422 antibody
    • OTTHUMP00000225477 antibody
    • OTTHUMP00000225545 antibody
    • OTTHUMP00000230603 antibody
    • OTTHUMP00000230605 antibody
    • pro-neuregulin-1, membrane-bound isoform antibody
    • Pro-NRG1 antibody
    • ProNRG1 antibody
    • Sensory and motor neuron-derived factor antibody
    • SMDF antibody
    see all

Anti-NRG1 antibody [7D5] images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue, staining NRG1 with ab2369.

References for Anti-NRG1 antibody [7D5] (ab2369)

ab2369 has not yet been referenced specifically in any publications.

Product Wall


According to the lab, the only information they have about the immunogen for the ab2369 antibody is Recombinant extracellular domain of rat NRG1 protein.


According to the Uniprot database for the rat protein (http://www.uniprot.org/u...

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Thank you for your interest in ab2369.

I'msorry but we do not have any images available demonstrating the use of this antibody in IHC or western blotting. We do guarantee our antibodies to work as noted on the online datasheet, however. So in...

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Unfortunately, the epitope has not been determined. Therefore, we do not know ab2369 cross reactivity with various isoforms.

Thank you for your enquiry. We have only the following information regarding the immunogen used to produce this antibody: Clone 7D5 was derived by immunizing BALB/c mice with recombinant extracellular domain of rat heregulin protein and fusing th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"