Overview

  • Product name
  • Description
    Goat polyclonal to NRG3
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    RNEIQRDSALTK

    , corresponding to amino acids 685-696 of Human NRG3 (NP_001010848.2)

  • Positive control
    • Human brain (cerebellum) lysate, mouse and rat brain lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/64000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 78 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
    • Tissue specificity
      Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
    • Sequence similarities
      Belongs to the neuregulin family.
      Contains 1 EGF-like domain.
    • Developmental stage
      Isoform 3 is expressed in fetal brain but not in other fetal tissues.
    • Domain
      The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
      ERBB receptor binding is elicited entirely by the EGF-like domain.
    • Post-translational
      modifications
      Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
      Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
    • Cellular localization
      Secreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
    • Information by UniProt
    • Database links
    • Alternative names
      • HRG3 antibody
      • Neuregulin 3 antibody
      • Neuregulin 3 like polypeptide antibody
      • Neuregulin-3 antibody
      • Neuregulin3 antibody
      • NRG-3 antibody
      • Nrg3 antibody
      • NRG3_HUMAN antibody
      • Pro neuregulin 3 antibody
      • pro NRG3 antibody
      • Pro-NRG3 antibody
      see all

    Anti-NRG3 antibody images

    • Anti-NRG3 antibody (ab77597) at 0.3 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg

      Predicted band size : 78 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-NRG3 antibody (ab77597)

    ab77597 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab77597.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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