Overview

  • Product nameAnti-NRG3 antibody
    See all NRG3 primary antibodies
  • Description
    Goat polyclonal to NRG3
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog, Chimpanzee
  • Immunogen

    Synthetic peptide:

    RNEIQRDSALTK

    , corresponding to amino acids 685-696 of Human NRG3 (NP_001010848.2)

  • Positive control
    • Human brain (cerebellum) lysate, mouse and rat brain lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/64000.
    WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 78 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDirect ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
    • Tissue specificityHighly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the neuregulin family.
      Contains 1 EGF-like domain.
    • Developmental stageIsoform 3 is expressed in fetal brain but not in other fetal tissues.
    • DomainThe cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
      ERBB receptor binding is elicited entirely by the EGF-like domain.
    • Post-translational
      modifications
      Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
      Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
    • Cellular localizationSecreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
    • Information by UniProt
    • Database links
    • Alternative names
      • HRG3 antibody
      • Neuregulin 3 antibody
      • Neuregulin 3 like polypeptide antibody
      • Neuregulin-3 antibody
      • Neuregulin3 antibody
      • NRG-3 antibody
      • Nrg3 antibody
      • NRG3_HUMAN antibody
      • Pro neuregulin 3 antibody
      • pro NRG3 antibody
      • Pro-NRG3 antibody
      see all

    Anti-NRG3 antibody images

    • Anti-NRG3 antibody (ab77597) at 0.3 µg/ml + human brain (cerebellum) lysate in RIPA buffer at 35 µg

      Predicted band size : 78 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-NRG3 antibody (ab77597)

    ab77597 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab77597.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"