Anti-NRG3 antibody [EPR4148] (ab109256)

Overview

  • Product nameAnti-NRG3 antibody [EPR4148]
    See all NRG3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4148] to NRG3
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the cytoplasmic domain of Human NRG3.

  • Positive control
    • Fetal brain, SH-SY5Y, C6, mouse brain, and rat brain lysates; Permeabilized C6 cells
  • General notes

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 77 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    • FunctionDirect ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.
    • Tissue specificityHighly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, colon and peripheral blood leukocytes.
    • Sequence similaritiesBelongs to the neuregulin family.
      Contains 1 EGF-like domain.
    • Developmental stageIsoform 3 is expressed in fetal brain but not in other fetal tissues.
    • DomainThe cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization.
      ERBB receptor binding is elicited entirely by the EGF-like domain.
    • Post-translational
      modifications
      Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form.
      Extensive glycosylation precedes the proteolytic cleavage (By similarity). Isoform 3 is glycosylated.
    • Cellular localizationSecreted; Cell membrane. Does not seem to be active and Cell membrane. Isoform 3 is also proteolytically released as a soluble form.
    • Information by UniProt
    • Database links
    • Alternative names
      • HRG3 antibody
      • Neuregulin 3 antibody
      • Neuregulin 3 like polypeptide antibody
      • Neuregulin-3 antibody
      • Neuregulin3 antibody
      • NRG-3 antibody
      • Nrg3 antibody
      • NRG3_HUMAN antibody
      • Pro neuregulin 3 antibody
      • pro NRG3 antibody
      • Pro-NRG3 antibody
      see all

    Anti-NRG3 antibody [EPR4148] images

    • All lanes : Anti-NRG3 antibody [EPR4148] (ab109256) at 1/1000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : C6 lysate
      Lane 4 : Mouse brain lysate
      Lane 5 : Rat brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 77 kDa
      Observed band size : 56 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized C6 cells using ab109256 (red) or a rabbit IgG (negative) (green).

    References for Anti-NRG3 antibody [EPR4148] (ab109256)

    ab109256 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (brain)
    Specification brain
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Tanveer Ahmed

    Verified customer

    Submitted Jul 15 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"