The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/100.
Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
Belongs to the neuregulin family. Contains 1 EGF-like domain.
The cytoplasmic domain may be involved in the regulation of trafficking and proteolytic processing. Regulation of the proteolytic processing involves initial intracellular domain dimerization. ERBB receptor binding is elicited entirely by the EGF-like domain.
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. Extensive glycosylation precedes the proteolytic cleavage.
Secreted and Cell membrane. Does not seem to be active.
Ab60090 (1/20 dilutio) staining NRG4 in formalin fixed, paraffin embedded human cancer tissue sections by immunohistochemistry. ab60090 was peroxidase conjugated to the secondary antibody, followed by AEC staining.
BC = breast carcinoma.