The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 59 kDa).
FunctionLigand-dependent coactivator of nuclear receptors. Enhance transcriptional activity of the nuclear receptors NR3C1 and AR. May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Tissue specificityHighly expressed in skeletal muscle and testis. Expressed to a lesser degree in heart, prostate, and adrenal gland.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 IQ domain. Contains 7 WD repeats.