Overview

  • Product name
  • Description
    Rabbit polyclonal to NSBP1
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab56031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22420896
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the HMGN family.
  • Domain
    Specifically targeted by its C-terminus to nucleosomes in euchromatin.
  • Cellular localization
    Nucleus. Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • High mobility group nucleosome binding domain 5 antibody
    • High mobility group nucleosome-binding domain-containing protein 5 antibody
    • Hmgn5 antibody
    • HMGN5_HUMAN antibody
    • NBP 45 antibody
    • NSBP 1 antibody
    • NSBP1 antibody
    • Nucleosomal binding protein 1 antibody
    • Nucleosome binding protein 1 antibody
    • Nucleosome-binding protein 1 antibody
    see all

Images

  • Anti-NSBP1 antibody (ab56031) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 32 kDa

References

This product has been referenced in:
  • Main H  et al. Notch signaling maintains neural rosette polarity. PLoS One 8:e62959 (2013). IHC ; Mouse . Read more (PubMed: 23675446) »
  • Ji SQ  et al. Knockdown of the nucleosome binding protein 1 inhibits the growth and invasion of clear cell renal cell carcinoma cells in vitro and in vivo. J Exp Clin Cancer Res 31:22 (2012). IHC-P ; Human . Read more (PubMed: 22420896) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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