Anti-NSD3 antibody [2E9] (ab66745)


  • Product nameAnti-NSD3 antibody [2E9]
    See all NSD3 primary antibodies
  • Description
    Mouse monoclonal [2E9] to NSD3
  • Tested applicationsSuitable for: WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to amino acids 383-660 of Human NSD3 purified from E. coli

  • Positive control
    • HeLa cell lysate
  • General notes

    ab66745, clone 2E9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human NSD3 protein.



Our Abpromise guarantee covers the use of ab66745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Detects a band of approximately 72 kDa. (predicted molecular weight for the full length protein: 162 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • FunctionHistone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
  • Tissue specificityHighly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
  • Involvement in diseaseDefects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
    A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
  • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp667H044 antibody
    • FLJ20353 antibody
    • Histone lysine N methyltransferase NSD3 antibody
    • Histone-lysine N-methyltransferase NSD3 antibody
    • MGC126766 antibody
    • MGC142029 antibody
    • NSD 3 antibody
    • NSD3_HUMAN antibody
    • Nuclear set domain containing 3 antibody
    • Nuclear SET domain containing protein 3 antibody
    • Nuclear SET domain-containing protein 3 antibody
    • pp14328 antibody
    • Protein whistle antibody
    • Whistle antibody
    • WHSC1 like protein 1 antibody
    • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody
    • WHSC1-like protein 1 antibody
    • WHSC1L1 antibody
    • WHSC1L1 protein antibody
    • Wolf Hirschhorn syndrome candidate 1 like 1 antibody
    • Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody
    see all

Anti-NSD3 antibody [2E9] images

  • Overlay histogram showing HeLa cells stained with ab66745 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab66745, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-NSD3 antibody [2E9] (ab66745) at 1/2000 dilution + HeLa cell lysate at 40 µg

    HRP-conjugated goat anti-mouse
    developed using the ECL technique

    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

References for Anti-NSD3 antibody [2E9] (ab66745)

ab66745 has not yet been referenced specifically in any publications.

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I have been in touch with the supplier regading thisproduct. Unfortunatley they have not tested to determine wheter this reacts with each isoform of NSD3 and cannot provide any further immunogen information. I ...

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