Overview

  • Product nameAnti-NSD3 antibody
    See all NSD3 primary antibodies
  • Description
    Rabbit polyclonal to NSD3
  • SpecificityThis antibody should recognise both the 72 and 161 kDa isoforms: NP_060248 (645 aa) - 72.6 kDa, NP_075447 (1437 aa) - 161 kDa.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from residues 500 - 600 of Human NSD3.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa, HEK293.

      This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Human Breast Carcinoma.

      This antibody gave a positive signal in the following cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab4514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72.6, 161 kDa (predicted molecular weight: 72.6 , 161 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • FunctionHistone methyltransferase. Preferentially methylates 'Lys-4' and 'Lys-27' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression.
  • Tissue specificityHighly expressed in brain, heart and skeletal muscle. Expressed at lower level in liver and lung.
  • Involvement in diseaseDefects in WHSC1L1 may be involved in non small cell lung carcinomas (NSCLC). Amplified or overexpressed in NSCLC.
    A chromosomal aberration involving WHSC1L1 is found in childhood acute myeloid leukemia. Translocation t(8;11)(p11.2;p15) with NUP98.
  • Sequence similaritiesBelongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 4 PHD-type zinc fingers.
    Contains 1 post-SET domain.
    Contains 2 PWWP domains.
    Contains 1 SET domain.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp667H044 antibody
    • FLJ20353 antibody
    • Histone lysine N methyltransferase NSD3 antibody
    • Histone-lysine N-methyltransferase NSD3 antibody
    • MGC126766 antibody
    • MGC142029 antibody
    • NSD 3 antibody
    • NSD3_HUMAN antibody
    • Nuclear set domain containing 3 antibody
    • Nuclear SET domain containing protein 3 antibody
    • Nuclear SET domain-containing protein 3 antibody
    • pp14328 antibody
    • Protein whistle antibody
    • Whistle antibody
    • WHSC1 like protein 1 antibody
    • WHSC1-like 1 isoform 9 with methyltransferase activity to lysine antibody
    • WHSC1-like protein 1 antibody
    • WHSC1L1 antibody
    • WHSC1L1 protein antibody
    • Wolf Hirschhorn syndrome candidate 1 like 1 antibody
    • Wolf-Hirschhorn syndrome candidate 1-like protein 1 antibody
    see all

Anti-NSD3 antibody images

  • Breast carcinoma tissue was fixed in 10% formalin at 4°C overnight and paraffin embedded. Following blocking the tissue sections were incubated with ab4514 (1/50 dilution) at 37°C for 1 hour, and with the secondary antibody at 37°C for 30 minutes. The slides were counterstained with Hematoxylin. Washes were carried out using TBS. Heat mediated antigen retrieval was carried out using the Microwave method before commencing the staining protocol. ab4514 staining appears to be localized mainly to the cytoplasm. Although the putative cellular localization of NSD3 is the nucleus we have found no evidence in the current literature to confirm this and we believe ab4514 may be detecting a cytoplasmic form of the protein. We would be very grateful for any customer feedback about NSD3 and ab4514.


  • All lanes : Anti-NSD3 antibody (ab4514) at 1 µg/ml

    Lane 1 : HeLa whole cell extract
    Lane 2 : HEK293 cell extract
    Lane 3 : HeLa whole cell extract with Human NSD3 peptide (ab14995) at 1 µg/ml
    Lane 4 : HEK293 cell extract with Human NSD3 peptide (ab14995) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 72.6 , 161 kDa
    Observed band size : 72.6,161 kDa
    Additional bands at : 115 kDa (possible cross reactivity).
  • ICC/IF image of ab4514 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4514, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    As seen in Immunohistochemical analysis (shown above), although the putative cellular localization of NSD3 is the nucleus we have found no evidence in the current literature to confirm this and we believe ab4514 may be detecting a cytoplasmic form of the protein.

References for Anti-NSD3 antibody (ab4514)

This product has been referenced in:
  • Rahman S  et al. The Brd4 extraterminal domain confers transcription activation independent of pTEFb by recruiting multiple proteins, including NSD3. Mol Cell Biol 31:2641-52 (2011). Read more (PubMed: 21555454) »

See 1 Publication for this product

Product Wall

Abreviews
Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Human Cell lysate - nuclear (Hela)
Specification Hela
Total protein in input 4e+007 cells
Username

Abcam user community

Verified customer

Submitted Jun 03 2013

Thank you for confirming this with customer.

I have contacted the laboratory for checking if they are happy to send a free vial to this customer. I will contact you soon with more updates.

Though I will be able to sort this case this...

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Thank you for contacting us.

Unfortunately we have no stock available at the moment. We do have an lot in characterisation which has passed but our WB band is running higher than expected. Our scientists are working with collaborators to find ...

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Thank you very much for your call yesterday and for letting us know about the trouble with ab4514.

As we discussed, I'm sending a free of charge vial of the HeLa cell lysate, ab29545, on the order *** which should arrive today.

Plea...

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Thank you for getting back to me with further information. I have passed on the details to my business development colleagues and we will discuss the possibility of starting an immunisation program against this target. We are always keen to get...

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