Anti-NSE antibody [1C1] (HRP) (ab24472)


  • Product name
    Anti-NSE antibody [1C1] (HRP)
    See all NSE primary antibodies
  • Description
    Mouse monoclonal [1C1] to NSE (HRP)
  • Conjugation
  • Specificity
    Reacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Neuron Specific Enolase from human brain.



Our Abpromise guarantee covers the use of ab24472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/1000.


  • Function
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificity
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • Pathway
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similarities
    Belongs to the enolase family.
  • Developmental stage
    During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localization
    Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • neuronal enriched enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [1C1] (HRP) images

  • All lanes : Anti-NSE antibody [1C1] (HRP) (ab24472) at 1/1000 dilution

    Lane 2 : Recombinant Human NSE protein (ab78797) at 5 µg
    Lane 3 : Recombinant Human NSE protein (ab78797) at 0.005 µg
    Lane 4 : Whole tissue lysate prepared from human brain at 15 µg

    Developed using the ECL technique

    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

    Exposure time : 1 minute

    Image courtesy of Dr Rachel Barker by Abreview.

    See Abreview

References for Anti-NSE antibody [1C1] (HRP) (ab24472)

ab24472 has not yet been referenced specifically in any publications.

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Thank you for contacting us. I am sorry to hear that you are having trouble using this antibody.

I have read the details provided and suspect that the standard may not be pure enough or may be high conc. of . standard is needed. I have furthe...

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Thank you for your enquiry.

I have investigated our records for ab24472 with my colleagues and the originator. I can confirm that regrettably there is an error on the datasheet, and this antibody does not contain sodium azide.


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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (brain)
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20% Tris-HCl)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Dr. Rachel Barker

Verified customer

Submitted Mar 21 2012


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