Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)

Overview

  • Product nameAnti-NSE antibody [1C1] (HRP) - Neuronal MarkerSee all NSE primary antibodies ...
  • Description
    Mouse monoclonal [1C1] to NSE - Neuronal Marker (HRP)
  • ConjugationHRP
  • SpecificityReacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
  • Tested applicationsELISA, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Neuron Specific Enolase from human brain.

Applications

Our Abpromise guarantee covers the use of ab24472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [5A4] to NSE - Neuronal Marker (ab10169) and Mouse monoclonal [5E2] to NSE - Neuronal Marker (ab10171) and Mouse monoclonal [5G10] to NSE - Neuronal Marker (ab8324). Can be used as detection antibody in conjunction with recommended pair(s).
WB 1/1000.

Target

  • FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Target information above from: UniProt accession P09104 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • Eno2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [1C1] (HRP) - Neuronal Marker images

  • All lanes : Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472) at 1/1000 dilution

    Lane 2 : Human NSE full length protein (ab78797) at 5 µg
    Lane 3 : Human NSE full length protein (ab78797) at 0.005 µg
    Lane 4 : Whole tissue lysate prepared from human brain at 15 µg

    developed using the ECL technique

    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of Dr Rachel Barker by Abreview.

    See Abreview

References for Anti-NSE antibody [1C1] (HRP) - Neuronal Marker (ab24472)

ab24472 has not yet been referenced specifically in any publications.

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I have investigated our records for ab24472 with my colleagues and the originator. I can confirm that regrettably there is an error on the datasheet, and this antibody does not contain sodium azide.

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I have read the details provided and suspect that the standard may not be pure enough or may be high conc. of . standard is needed. I have furthe...

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Application Western blot
Sample Human Tissue lysate - whole (brain)
Loading amount 15 µg
Specification brain
Gel Running Conditions Reduced Denaturing (4-20% Tris-HCl)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Dr. Rachel Barker

Verified customer

Submitted Mar 21 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"