Overview

  • Product nameAnti-NSE antibody [5G10]
    See all NSE primary antibodies
  • Description
    Mouse monoclonal [5G10] to NSE
  • SpecificityReacts with 45 kD band corresponding to NSE in immunoblotting of human brain extracts
  • Tested applicationsSuitable for: Sandwich ELISA, ICC/IF, ELISA, IHC-FoFr, IHC-P, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Neuron Specific Enolase from human brain

Properties

Applications

Our Abpromise guarantee covers the use of ab8324 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [1C1] to NSE (ab8328). For sandwich ELISA, we recommend using this antibody for capture with ab24472 as detection. Can be used as capture antibody in conjunction with ab8328 and ab24472.
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration. Can be paired for ELISA with Mouse monoclonal [1C1] to NSE (ab8328).
IHC-FoFr 1/100 - 1/300.
IHC-P Use at an assay dependent concentration. Recommended dilution for immunohistochemistry on formalin-fixed and paraffin-embedded sections is 1:100 - 1:300. Optimal dilution must be determined by experimentation.
Flow Cyt Use 1-2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 24125751

Target

  • FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody [5G10] images

  • Overlay histogram showing SH-SY5Y cells stained with ab8324 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8324, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-NSE antibody [5G10] (ab8324)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Cross reactivity of ab8324 with mouse has not been tested. I shall forward a datasheet for ab8197, another Mouse monoclonal to Neuron Specific Enolase (NSE) - where cross-reactivity has shown with guinea pigs. However, as this antibody cross reacts wit...

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