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Recombinant full length protein purified from E.coli (Human).
Our Abpromise guarantee covers the use of ab16808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. (paraformaldehyde fixed cells)
ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-FoFr||1/100. PubMed: 19279043|
|ICC/IF||Use a concentration of 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 48 kDa.|
ICC/IF image of ab16808 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16808 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.