The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
1/500. For optimal signal, fix with 3.7% formalin in PBS for one minute followed by 100% cold (-20C) methanol for one minute.
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
Belongs to the enolase family.
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
Immunofluorescent staining of human embryonic kidney cell line 293 by ab64721 (1:500). Hek293 expresses many neuronal proteins.
The red channel shows staining with ab64721, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with a monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1), ab64722. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.