Overview

  • Product nameAnti-NSE antibody
    See all NSE primary antibodies
  • Description
    Rabbit polyclonal to NSE
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length human NSE purified from E. coli.

  • Positive control
    • Rat spinal cord or human embryonic kidney cells line 293.
  • General notesThe total protein content in serum: about 60mg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab64721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF 1/500. For optimal signal, fix with 3.7% formalin in PBS for one minute followed by 100% cold (-20C) methanol for one minute.

Target

  • FunctionHas neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • Tissue specificityThe alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • PathwayCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • Sequence similaritiesBelongs to the enolase family.
  • Developmental stageDuring ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • Cellular localizationCytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2 phospho D glycerate hydrolyase antibody
    • 2-phospho-D-glycerate hydro-lyase antibody
    • Eno 2 antibody
    • ENO2 antibody
    • ENOG antibody
    • ENOG_HUMAN antibody
    • Enolase 2 (gamma, neuronal) antibody
    • Enolase 2 antibody
    • Enolase 2 gamma neuronal antibody
    • Enolase2 antibody
    • Epididymis secretory protein Li 279 antibody
    • Gamma enolase antibody
    • Gamma-enolase antibody
    • HEL S 279 antibody
    • Neural enolase antibody
    • Neuron specific enolase antibody
    • Neuron specific gamma enolase antibody
    • Neuron-specific enolase antibody
    • Neurone specific enolase antibody
    • NSE antibody
    see all

Anti-NSE antibody images

  • Anti-NSE antibody (ab64721) at 1/2000 dilution + Rat spinal cord

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
  • Immunofluorescent staining of human embryonic kidney cell line 293 by ab64721 (1:500). Hek293 expresses many neuronal proteins.

    The red channel shows staining with ab64721, which recognizes all of these 293 cells. The green channels shows staining for another neuronal marker with a monoclonal antibody to ubiquitin C-terminal hydrolase 1 (UCHL1), ab64722. This neuronal gene is apparently activated in a cell density dependent fashion and at this stage only a few cells express this protein. However all cells that express NSE also express UCHL1.

References for Anti-NSE antibody (ab64721)

ab64721 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab64721.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"