Overview

  • Product name
  • Description
    Mouse polyclonal to NSE2
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Full length protein, corresponding to amino acids 1-247 of Human NSE2 (Accession number: NP_775956; UniProt ID: Q96MF7).

  • Positive control
    • NSE2 transfected 293T cell lysate; HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab169355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
  • Pathway
    Protein modification; protein sumoylation.
  • Sequence similarities
    Belongs to the NSE2 family.
    Contains 1 SP-RING-type zinc finger.
  • Post-translational
    modifications
    Sumoylated, possibly via autosumoylation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • C8orf36 antibody
    • E3 SUMO-protein ligase NSE2 antibody
    • hMMS21 antibody
    • hNSE2 antibody
    • methyl methanesulfonate sensitivity gene 21 antibody
    • MMS21 antibody
    • MMS21 homolog antibody
    • non-SMC element 2 antibody
    • Non-SMC element 2 homolog antibody
    • Non-structural maintenance of chromosomes element 2 homolog antibody
    • NSE2_HUMAN antibody
    • Nsmce2 antibody
    see all

Images

  • All lanes : Anti-NSE2 antibody (ab169355) at 1 µg/ml

    Lane 1 : NSE2 transfected 293T cell lysate

    Lane 2 : Non-transfected 293T cell lysate


    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 28 kDa

  • Immunofluorescent analysis of HeLa cells labeling NSE2 with ab169355 at 10 µg/ml.

References

ab169355 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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