Overview

  • Product name
  • Description
    Rabbit polyclonal to NSE2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human NSE2.

    (Peptide available as ab94387.)

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates: Caco2; HepG2; A431.

Properties

Applications

Our Abpromise guarantee covers the use of ab64683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 28 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair.
    • Pathway
      Protein modification; protein sumoylation.
    • Sequence similarities
      Belongs to the NSE2 family.
      Contains 1 SP-RING-type zinc finger.
    • Post-translational
      modifications
      Sumoylated, possibly via autosumoylation.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C8orf36 antibody
      • E3 SUMO-protein ligase NSE2 antibody
      • hMMS21 antibody
      • hNSE2 antibody
      • methyl methanesulfonate sensitivity gene 21 antibody
      • MMS21 antibody
      • MMS21 homolog antibody
      • non-SMC element 2 antibody
      • Non-SMC element 2 homolog antibody
      • Non-structural maintenance of chromosomes element 2 homolog antibody
      • NSE2_HUMAN antibody
      • Nsmce2 antibody
      see all

    Images

    • All lanes : Anti-NSE2 antibody (ab64683) at 1 µg/ml

      Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
      Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
      Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunizing peptide at 1 µg/ml
      Lane 4 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
      Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 6 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 28 kDa
      Observed band size : 56 kDa (why is the actual band size different from the predicted?)


      Exposure time : 20 minutes

    References

    ab64683 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab64683.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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